{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T13:07:19Z","timestamp":1787663239270,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,12,9]],"date-time":"2016-12-09T00:00:00Z","timestamp":1481241600000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"Dopamine (DA<\/jats:styled-content>) has emerged as a potent inhibitory neuromodulator of luteinsing hormone (LH)<\/jats:styled-content> secretion and reproduction in teleosts. The DA<\/jats:styled-content> neurones located in the anterior subdivision of nucleus preopticus periventricularis (NPP<\/jats:styled-content>a) in the preoptic area (POA<\/jats:styled-content>) innervate the pituitary gland and regulate LH<\/jats:styled-content> cells. Although a reduction in the inhibitory DA<\/jats:styled-content>ergic tone is crucial for stimulatory action of gonadotrophin\u2010releasing hormone (GnRH)<\/jats:styled-content> on LH<\/jats:styled-content> cells, the role of other hypothalamic factors is suggested but not fully understood. Nonapeptide, isotocin (IST<\/jats:styled-content>) has emerged as a likely candidate that may also influence the LH<\/jats:styled-content> cell function. IST<\/jats:styled-content> neurones reside in the nucleus preopticus and innervate LH<\/jats:styled-content> cells. While IST<\/jats:styled-content> treatment dramatically elevated LH<\/jats:styled-content> secretion, the IST<\/jats:styled-content> levels in brain peaked during spawning. In a pilot study on the catfish, Clarias batrachus<\/jats:italic>, we observed a dense network of IST<\/jats:styled-content>\u2010immunoreactive (IST\u2010IR) fibres in the NPP<\/jats:styled-content>a, the region known to harbour hypophysiotropic DA<\/jats:styled-content> neurones. Application of the double immunofluorescence method showed a dense IST<\/jats:styled-content>\u2010IR fibre network around the tyrosine hydroxylase\u2010immunoreactive (TH\u2010IR) neurones in the NPP<\/jats:styled-content>a region. A great majority of the TH<\/jats:styled-content>\u2010IR neurones in the NPP<\/jats:styled-content>a were contacted by IST<\/jats:styled-content>\u2010IR fibres during the spawning phase. The NPP<\/jats:styled-content>a therefore appears to be a site for the intense interaction of DA<\/jats:styled-content> and IST<\/jats:styled-content>. IST<\/jats:styled-content>\u2010IR fibre innervation in NPP<\/jats:styled-content>a showed reproduction phase\u2010dependent changes. The percent fluorescent area of IST<\/jats:styled-content>\u2010IR fibres showed a gradual increase from the resting through prespawning phases (resting: 7.5\u00a0\u00b1\u00a01.04; preparatory: 8.6\u00a0\u00b1\u00a00.8; prespawning: 15.5\u00a0\u00b1\u00a01.4), reaching a peak in the spawning phase (28\u00a0\u00b1\u00a02.3; P\u00a0<\u00a00.001). Compared to the spawning phase, a drastic reduction in IST<\/jats:styled-content>\u2010IR fibres in the NPP<\/jats:styled-content>a was observed during the postspawning phase (8.4\u00a0\u00b1\u00a00.9; P\u00a0<\u00a00.001). Superfused slices of the POA<\/jats:styled-content> of C.\u00a0batrachus<\/jats:italic> treated with IST<\/jats:styled-content> peptide resulted in a significant reduction in TH<\/jats:styled-content> immunoreactivity in the NPP<\/jats:styled-content>a (Control: 45.3\u00a0\u00b1\u00a04.2; IST<\/jats:styled-content> peptide, 5\u00a0\u03bcm<\/jats:sc>: 29.4\u00a0\u00b1\u00a04.7; P\u00a0<\u00a00.05). We suggest that the intense interaction between IST<\/jats:styled-content> and DA<\/jats:styled-content> in the NPP<\/jats:styled-content>a, most probably of an inhibitory nature, may be critical for the regulation of LH<\/jats:styled-content> cells and reproduction in teleosts.<\/jats:p>","DOI":"10.1111\/jne.12441","type":"journal-article","created":{"date-parts":[[2016,12,15]],"date-time":"2016-12-15T05:18:50Z","timestamp":1481779130000},"source":"Crossref","is-referenced-by-count":7,"title":["Role of Isotocin in the Regulation of the Hypophysiotropic Dopamine Neurones in the Preoptic Area of the Catfish, Clarias batrachus<\/i>"],"prefix":"10.1111","volume":"28","author":[{"given":"O.","family":"Singh","sequence":"first","affiliation":[{"name":"School of Biological Sciences National Institute of Science Education and Research (NISER)\u2010Bhubaneswar Jatni Odisha India"},{"name":"Training School Complex Homi Bhabha National Institute Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Kumar","sequence":"additional","affiliation":[{"name":"School of Biological Sciences National Institute of Science Education and Research (NISER)\u2010Bhubaneswar Jatni Odisha India"},{"name":"Training School Complex Homi Bhabha National Institute Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.","family":"Singh","sequence":"additional","affiliation":[{"name":"School of Biological Sciences National Institute of Science Education and Research (NISER)\u2010Bhubaneswar Jatni Odisha India"},{"name":"Training School Complex Homi Bhabha National Institute Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Bhute","sequence":"additional","affiliation":[{"name":"Department of Zoology DRB Sindhu Mahavidyalaya Nagpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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