{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T14:19:56Z","timestamp":1777990796306,"version":"3.51.4"},"reference-count":85,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,4,26]],"date-time":"2017-04-26T00:00:00Z","timestamp":1493164800000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology , Ministry of Science and Technology","doi-asserted-by":"publisher","award":["BT\/PR4909\/MED\/30\/746\/2012"],"award-info":[{"award-number":["BT\/PR4909\/MED\/30\/746\/2012"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology , Ministry of Science and Technology","doi-asserted-by":"publisher","award":["BT\/PR6325\/GBD\/27\/402\/2012"],"award-info":[{"award-number":["BT\/PR6325\/GBD\/27\/402\/2012"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2017,4]]},"abstract":"Recent studies suggest an important role for transient receptor potential vanilloid (TRPV<\/jats:styled-content>) ion channels in neural and neuroendocrine regulation. The TRPV<\/jats:styled-content> subfamily consists of six members: TRPV<\/jats:styled-content>1\u20106. While the neuroanatomical and functional correlates of TRPV<\/jats:styled-content>1\u20104 have been studied extensively, relevant information about TRPV<\/jats:styled-content>5 and TRPV<\/jats:styled-content>6, which are highly selective for Ca2+<\/jats:sup>, is limited. We detected TRPV<\/jats:styled-content>5 mRNA<\/jats:styled-content> expression in the olfactory bulb, cortex, hypothalamus, hippocampus, midbrain, brainstem and cerebellum of the rat. TRPV<\/jats:styled-content>5\u2010immunoreactive neurones were conspicuously seen in the hypothalamic paraventricular (PVN<\/jats:styled-content>), supraoptic (SON<\/jats:styled-content>), accessory neurosecretory (ANS<\/jats:styled-content>), supraoptic nucleus, retrochiasmatic part (SOR), arcuate (ARC<\/jats:styled-content>) and medial tuberal nuclei, hippocampus, midbrain, brainstem and cerebellum. Glial cells also showed TRPV<\/jats:styled-content>5\u2010immunoreactivity. To test the neuroendocrine relevance of TRPV<\/jats:styled-content>5, we focused on vasopressin, oxytocin and cocaine\u2010 and amphetamine\u2010regulated transcript (CART<\/jats:styled-content>) as representative candidate markers with which TRPV<\/jats:styled-content>5 may co\u2010exist. In the hypothalamic neurones, co\u2010expression of TRPV<\/jats:styled-content>5 was observed with vasopressin (PVN<\/jats:styled-content>: 50.73\u00b13.82%; SON<\/jats:styled-content>: 75.91\u00b12.34%; ANS<\/jats:styled-content>: 49.12\u00b14.28%; SOR<\/jats:styled-content>: 100%) and oxytocin (PVN<\/jats:styled-content>: 6.88\u00b11.21; SON<\/jats:styled-content>: 63.34\u00b15.69%; ANS<\/jats:styled-content>: 20.4\u00b14.14; SOR<\/jats:styled-content>: 86.5\u00b11.74%). While ARC<\/jats:styled-content> neurones express oestrogen receptors, 17\u03b2\u2010oestradiol regulates TRPV<\/jats:styled-content>5, as well as CART<\/jats:styled-content> neurones and astrocytes, in the ARC<\/jats:styled-content>. Furthermore, ARC CART<\/jats:styled-content> neurones are known to project to the preoptic area, and innervate and regulate GnRH<\/jats:styled-content> neurones. Using double\u2010immunofluorescence, glial fibrillary acidic protein<\/jats:styled-content>\u2010labelled astrocytes and the majority of CART<\/jats:styled-content> neurones in the ARC<\/jats:styled-content> showed TRPV<\/jats:styled-content>5\u2010immunoreactivity. Following iontophoresis of retrograde neuronal tracer, cholera toxin \u03b2 (CtB) into the anteroventral periventricular nucleus and median preoptic nucleus, retrograde accumulation of CtB was observed in most TRPV<\/jats:styled-content>5\u2010equipped ARC CART<\/jats:styled-content> neurones. Next, we determined the response of TRPV<\/jats:styled-content>5\u2010elements in the ARC<\/jats:styled-content> during the oestrous cycle. Compared to pro\u2010oestrus, a significant increase (P<\/jats:italic><.001) in the percentage of TRPV<\/jats:styled-content>5\u2010expressing CART<\/jats:styled-content> neurones was observed during oestrus, metoestrus, and dioestrus. TRPV<\/jats:styled-content>5\u2010immunoreactivity in the astrocytes, however, showed a significant increase during metoestrus and dioestrus. We suggest that the TRPV<\/jats:styled-content>5 ion channel may serve as an important regulator of neural and neuroendocrine pathways in the brain.<\/jats:p>","DOI":"10.1111\/jne.12466","type":"journal-article","created":{"date-parts":[[2017,2,24]],"date-time":"2017-02-24T21:36:42Z","timestamp":1487972202000},"source":"Crossref","is-referenced-by-count":20,"title":["Transient receptor potential vanilloid 5 (TRPV5), a highly Ca2+<\/sup>\u2010selective TRP<\/scp> channel in the rat brain: relevance to neuroendocrine regulation"],"prefix":"10.1111","volume":"29","author":[{"given":"S.","family":"Kumar","sequence":"first","affiliation":[{"name":"School of Biological Sciences National Institute of Science Education and Research (NISER) Bhubaneswar India"},{"name":"Homi Bhabha National Institute Training School Complex, Anushakti Nagar Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U.","family":"Singh","sequence":"additional","affiliation":[{"name":"School of Biological Sciences National Institute of Science Education and Research (NISER) Bhubaneswar India"},{"name":"Homi Bhabha National Institute Training School Complex, Anushakti Nagar Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Goswami","sequence":"additional","affiliation":[{"name":"School of Biological Sciences National Institute of Science Education and Research (NISER) Bhubaneswar India"},{"name":"Homi Bhabha National Institute Training School Complex, Anushakti Nagar Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3958-0204","authenticated-orcid":false,"given":"P. 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