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The<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>expression of these molecules was also determined using the reverse transcriptase\u2010polymerase chain reaction. The results obtained showed that, in WT adipose cells, all of the adenohypophyseal hormone receptors were present, with a higher<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>expression for growth hormone (<jats:styled-content style=\"fixed-case\">GH) receptor<\/jats:styled-content>and thyroid\u2010stimulating hormone (<jats:styled-content style=\"fixed-case\">TSH) receptor<\/jats:styled-content>. Of the total cells in the anterior pituitary lobe, 17.09\u00b10.9% were leptin receptor (<jats:styled-content style=\"fixed-case\">LEPR<\/jats:styled-content>) immunoreactive (\u2010IR), mainly in<jats:styled-content style=\"fixed-case\">GH<\/jats:styled-content>\u2010IR and prolactin (<jats:styled-content style=\"fixed-case\">PRL)<\/jats:styled-content>\u2010IR cells (41.5\u00b13.8%; 13.5\u00b11.7%, respectively). In<jats:italic>Dlk1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>mice, adipocyte cells showed a significant increase in the<jats:styled-content style=\"fixed-case\">TSH receptor mRNA<\/jats:styled-content>expression level. Moreover, the percentage of<jats:styled-content style=\"fixed-case\">LEPR<\/jats:styled-content>\u2010IR<jats:styled-content style=\"fixed-case\">GH<\/jats:styled-content>cells showed a statistically significant increase compared to controls, from 41.5\u00b13.8% to 53.1\u00b14.0%. By contrast, only 3.0\u00b10.6% of<jats:styled-content style=\"fixed-case\">LEP<\/jats:styled-content>\u2010IR anterior pituitary cells were detected in Dlk1 KO mice, as opposed to 6.8\u00b11.1% observed in<jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>mice. The results suggest that relationships exist between adipocytes and pituitary<jats:styled-content style=\"fixed-case\">GH<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\">PRL<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">TSH<\/jats:styled-content>cells, in addition to an influence with respect to the synthesis and release of pituitary leptin, particularly in<jats:styled-content style=\"fixed-case\">PRL<\/jats:styled-content>cells.<\/jats:p>","DOI":"10.1111\/jne.12507","type":"journal-article","created":{"date-parts":[[2017,7,18]],"date-time":"2017-07-18T04:06:53Z","timestamp":1500350813000},"source":"Crossref","is-referenced-by-count":11,"title":["Delta\u2010like protein 1 in the pituitary\u2010adipose axis in the adult male mouse"],"prefix":"10.1111","volume":"29","author":[{"given":"A. 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J.","family":"Gonz\u00e1lez\u2010G\u00f3mez","sequence":"additional","affiliation":[{"name":"Department of Inorganic and Organic Chemistry and Biochemistry School of Medicine\/Regional Centre for Biomedical Research Biomedicine Unit Spanish National Research Council\/University of Castilla\u2010La Mancha Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Gonz\u00e1lez\u2010G\u00f3mez","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences School of Medicine University of La Laguna Tenerife Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Laborda","sequence":"additional","affiliation":[{"name":"Department of Inorganic and Organic Chemistry and Biochemistry School of Medicine\/Regional Centre for Biomedical Research Biomedicine Unit Spanish National Research Council\/University of Castilla\u2010La Mancha Albacete 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