{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T11:35:04Z","timestamp":1778931304485,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,5,23]],"date-time":"2019-05-23T00:00:00Z","timestamp":1558569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although the melanin\u2010concentrating hormone (<jats:styled-content style=\"fixed-case\">MCH<\/jats:styled-content>) and its coding <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> are predominantly found in the tuberal hypothalamus, there is detectable synthesis of <jats:styled-content style=\"fixed-case\">MCH<\/jats:styled-content> in the preoptic hypothalamus exclusively in lactating dams, suggesting a participation of <jats:styled-content style=\"fixed-case\">MCH<\/jats:styled-content> in the alterations that take place after parturition. Also implicated in the dam physiology is oxytocin, a neurohormone released from the posterior pituitary that is necessary for milk ejection. Because the projection fields from oxytocin\u2010immunoreactive (\u2010IR) neurones and the mediobasal preoptic hypothalamus overlap and <jats:styled-content style=\"fixed-case\">MCH<\/jats:styled-content>\u2010IR neurones are found in proximity to oxytocin neurones, we investigated the spatial relationship between <jats:styled-content style=\"fixed-case\">MCH<\/jats:styled-content> and oxytocin fibres. Accordingly, we employed multiple immunohistochemistry labelling for <jats:styled-content style=\"fixed-case\">MCH<\/jats:styled-content> and oxytocin for light and electron microscopy techniques, in addition to i.v. tracer injection combined with in situ hybridisation to identify <jats:styled-content style=\"fixed-case\">MCH<\/jats:styled-content> neurones that project to neurosecretory areas. As described for other strains, lactating Long\u2010Evans dams also display immunoreactivity for <jats:styled-content style=\"fixed-case\">MCH<\/jats:styled-content> in the preoptic hypothalamus on days 12 and 19 of lactation. The appearance of these neurones is contemporaneous with an increase in <jats:styled-content style=\"fixed-case\">MCH<\/jats:styled-content>\u2010IR fibres in both the internal layer of the median eminence and the posterior pituitary. In both regions, <jats:styled-content style=\"fixed-case\">MCH<\/jats:styled-content>\u2010 and oxytocin\u2010IR fibres were found in great proximity, although there was no evidence for synaptic interaction between these two populations at the ultrastructural level. The tracer injection revealed that only mediobasal preoptic <jats:styled-content style=\"fixed-case\">MCH<\/jats:styled-content> neurones project to the posterior pituitary, suggesting a neuroendocrine\u2010modulatory role for this population. When taken together, the results obtained in the present study indicate that neuroplasticity events at the mediobasal preoptic hypothalamus that occur during late lactation may be part of a neuroendocrinology control loop involving both <jats:styled-content style=\"fixed-case\">MCH<\/jats:styled-content> and oxytocin.<\/jats:p>","DOI":"10.1111\/jne.12723","type":"journal-article","created":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T01:19:46Z","timestamp":1556673586000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Characterisation and origins of melanin\u2010concentrating hormone immunoreactive fibres of the posterior lobe of the pituitary and median eminence during lactation in the Long\u2010Evans rat"],"prefix":"10.1111","volume":"31","author":[{"given":"Helder C.","family":"Costa","sequence":"first","affiliation":[{"name":"Laboratory of Chemical Neuroanatomy Department of Anatomy Institute of Biomedical Sciences University of S\u00e3o Paulo (USP) S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joelcimar M.","family":"Da\u2010Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Chemical Neuroanatomy Department of Anatomy Institute of Biomedical Sciences University of S\u00e3o Paulo (USP) S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2020-2289","authenticated-orcid":false,"given":"Giovanne B.","family":"Diniz","sequence":"additional","affiliation":[{"name":"Laboratory of Chemical Neuroanatomy Department of Anatomy Institute of Biomedical Sciences University of S\u00e3o Paulo (USP) S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Livia C.","family":"Motta\u2010Teixeira","sequence":"additional","affiliation":[{"name":"Laboratory of Chemical Neuroanatomy Department of Anatomy Institute of Biomedical Sciences University of S\u00e3o Paulo (USP) S\u00e3o Paulo Brazil"},{"name":"Department of Physiology and Biophysics Institute of Biomedical Sciences University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata J.","family":"Da\u2010Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Chemical Neuroanatomy Department of Anatomy Institute of Biomedical Sciences University of S\u00e3o Paulo (USP) S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniella S.","family":"Battagello","sequence":"additional","affiliation":[{"name":"Laboratory of Chemical Neuroanatomy Department of Anatomy Institute of Biomedical Sciences University of S\u00e3o Paulo (USP) S\u00e3o Paulo Brazil"},{"name":"Center for Neuroscience and Behavior Institute of Psychology University of S\u00e3o Paulo (USP) S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciane V.","family":"Sita","sequence":"additional","affiliation":[{"name":"Laboratory of Chemical Neuroanatomy Department of Anatomy Institute of Biomedical Sciences University of S\u00e3o Paulo (USP) S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla","family":"de\u2010Moraes Machado","sequence":"additional","affiliation":[{"name":"Department of Anatomy Institute of Biosciences S\u00e3o Paulo State University (UNESP) Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 A. 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