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Previously, we have shown that rats overfed for the first 3\u00a0weeks of their neonatal life maintain obesity into adulthood. Neonatal overfeeding also leads to primed hypothalamic and hippocampal microglia that are hyper\u2010responsive to an immune challenge in adulthood. However, whether this microglial priming contributes to the obese phenotype and whether it is possible to reverse either the obesity or the microglial priming are not clear. In the present study, we hypothesised that an intervention with minocycline during the juvenile period (postnatal day 21\u201042) would normalise both the microglial priming and obesity. To induce obesity in neonatal Wistar rats, we manipulated the litter sizes in which they were suckled, yielding litters of 12 (control\u2010fed) or four (neonatally overfed). After weaning, we administered minocycline i.p. every second day for a 3\u2010week period and examined body composition and microglial profiles 24\u00a0hours following an immune challenge with lipopolysaccharide. As demonstrated previously, neonatal overfeeding resulted in prolonged weight gain. However, minocycline failed to reverse this effect. Minocycline did reverse microglial priming in feeding\u2010related regions of the hypothalamus, with minimal effects on pro\u2010inflammatory cytokines and on microglial number and morphology in the hippocampus. Thus, the programming effect of neonatal overfeeding on microglial priming can be ameliorated by minocycline later in life. However, the persistent obesity seen after neonatal overfeeding is likely not driven by changes in hypothalamic inflammation and microglial activity.<\/jats:p>","DOI":"10.1111\/jne.12757","type":"journal-article","created":{"date-parts":[[2019,6,25]],"date-time":"2019-06-25T14:00:40Z","timestamp":1561471240000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Obesity after neonatal overfeeding is independent of hypothalamic microgliosis"],"prefix":"10.1111","volume":"31","author":[{"given":"Alita","family":"Soch","sequence":"first","affiliation":[{"name":"School of Health and Biomedical Sciences RMIT University Melbourne VIC Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luba","family":"Sominsky","sequence":"additional","affiliation":[{"name":"School of Health and Biomedical Sciences RMIT University Melbourne VIC 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