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In the present study, the effects of <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content> and an inhibitor of the <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content> receptor, erlotinib, on <jats:styled-content style=\"fixed-case\">PRL<\/jats:styled-content> gene expression were examined both in the pituitary tumour cell line <jats:styled-content style=\"fixed-case\">GH<\/jats:styled-content>3 and in a primary culture of the mouse pituitary gland under similar experimental conditions. The results showed that <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content> stimulated <jats:styled-content style=\"fixed-case\">PRL<\/jats:styled-content> expression in <jats:styled-content style=\"fixed-case\">GH<\/jats:styled-content>3 cells, but not in normal cells. Erlotinib was found to counteract <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">GH<\/jats:styled-content>3 cells inhibiting the <jats:styled-content style=\"fixed-case\">PRL<\/jats:styled-content> expression enhanced by <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content>. By contrast, erlotinib induced an elevation in the <jats:styled-content style=\"fixed-case\">PRL mRNA<\/jats:styled-content> levels in the primary culture of the adult pituitary gland and the initiation of <jats:styled-content style=\"fixed-case\">PRL<\/jats:styled-content> production in the culture of the foetal pituitary gland in which <jats:styled-content style=\"fixed-case\">PRL<\/jats:styled-content> production had not yet occurred. Western blot analyses showed that <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content> induced and erlotinib inhibited the activation of extracellular regulated protein kinase equally in <jats:styled-content style=\"fixed-case\">GH<\/jats:styled-content>3 and normal cells. These results suggest that the consequences of <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content> receptor activation in normal <jats:styled-content style=\"fixed-case\">PRL<\/jats:styled-content> cells contradict those in adenomatous <jats:styled-content style=\"fixed-case\">PRL<\/jats:styled-content> cells.<\/jats:p>","DOI":"10.1111\/jne.12764","type":"journal-article","created":{"date-parts":[[2019,6,28]],"date-time":"2019-06-28T16:24:34Z","timestamp":1561739074000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Inhibition of epidermal growth factor receptor stimulates <scp>prolactin<\/scp> expression in primary culture of the mouse pituitary gland"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8349-8198","authenticated-orcid":false,"given":"Haruo","family":"Nogami","sequence":"first","affiliation":[{"name":"Department of Physical Therapy School of Health 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