{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T15:08:16Z","timestamp":1788188896355,"version":"build-2803163510"},"reference-count":88,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T00:00:00Z","timestamp":1573516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An injection of unesterified oestradiol (E<jats:sub>2<\/jats:sub>) facilitates receptive behaviour in E<jats:sub>2<\/jats:sub> benzoate (EB)\u2010primed, ovariectomised female rats when it is administered i.c.v. or systemically. The present study tested the hypothesis that inhibitors of protein kinase A (PKA), protein kinase G (PKG) or the Src\/mitogen\u2010activated protein kinase (MAPK) complex interfere with E<jats:sub>2<\/jats:sub> facilitation of receptive behaviour. In Experiment 1, lordosis induced by i.c.v. infusion of E<jats:sub>2<\/jats:sub> was significantly reduced by i.c.v. administration of Rp\u2010cAMPS, a PKA inhibitor, KT5823, a PKG inhibitor, and PP2 and PD98059, Src and MAPK inhibitors, respectively, between 30 and 240\u00a0minutes after infusion. In Experiment 2, we determined whether the ventromedial hypothalamus (VMH) is one of the neural sites at which those intracellular pathways participate in lordosis behaviour induced by E<jats:sub>2<\/jats:sub>. Administration of each of the four protein kinase inhibitors into the VMH blocked facilitation of lordosis induced by infusion of E<jats:sub>2<\/jats:sub> also into the VMH. These data support the hypothesis that activation of several protein kinase pathways is involved in the facilitation of lordosis by E<jats:sub>2<\/jats:sub> in EB\u2010primed rats.<\/jats:p>","DOI":"10.1111\/jne.12809","type":"journal-article","created":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T21:34:20Z","timestamp":1573594460000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Protein kinase inhibitors infused intraventricularly or into the ventromedial hypothalamus block short latency facilitation of lordosis by oestradiol"],"prefix":"10.1111","volume":"31","author":[{"given":"Raymundo","family":"Dom\u00ednguez\u2010Ord\u00f3\u00f1ez","sequence":"first","affiliation":[{"name":"Licenciatura en Ingenier\u00eda Agron\u00f3mica y Zootecnia Complejo Regional Centro Benem\u00e9rita Universidad Aut\u00f3noma de Puebla Tecamachalco Mexico"},{"name":"Department of Psychological and Brain Sciences Center for Neuroendocrine Studies University of Massachusetts Amherst MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos","family":"Garc\u00eda\u2010Ju\u00e1rez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Reproducci\u00f3n Animal Universidad Aut\u00f3noma de Tlaxcala\u2010CINVESTAV Tlaxcala M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco J.","family":"Lima\u2010Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Reproducci\u00f3n Animal Universidad Aut\u00f3noma de Tlaxcala\u2010CINVESTAV Tlaxcala M\u00e9xico"},{"name":"Facultad de Medicina Veterinaria y Zootecnia Universidad Aut\u00f3noma de Tlaxcala Tlaxcala M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Porfirio","family":"G\u00f3mora\u2010Arrati","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Reproducci\u00f3n Animal Universidad Aut\u00f3noma de Tlaxcala\u2010CINVESTAV Tlaxcala M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2145-0863","authenticated-orcid":false,"given":"Emilio","family":"Dom\u00ednguez\u2010Salazar","sequence":"additional","affiliation":[{"name":"\u00c1rea de Neurociencias Departamento de Biolog\u00eda de la Reproducci\u00f3n Universidad Aut\u00f3noma Metropolitana Iztapalapa M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ailyn","family":"Luna\u2010Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Reproducci\u00f3n Animal Universidad Aut\u00f3noma de Tlaxcala\u2010CINVESTAV Tlaxcala M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurt L.","family":"Hoffman","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Reproducci\u00f3n Animal Universidad Aut\u00f3noma de Tlaxcala\u2010CINVESTAV Tlaxcala M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey D.","family":"Blaustein","sequence":"additional","affiliation":[{"name":"Licenciatura en Ingenier\u00eda Agron\u00f3mica y Zootecnia Complejo Regional Centro Benem\u00e9rita Universidad Aut\u00f3noma de Puebla Tecamachalco Mexico"},{"name":"Department of Psychological and Brain Sciences Center for Neuroendocrine Studies University of Massachusetts Amherst MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne M.","family":"Etgen","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Albert Einstein College of Medicine Bronx NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1388-7088","authenticated-orcid":false,"given":"Oscar","family":"Gonz\u00e1lez\u2010Flores","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Reproducci\u00f3n Animal Universidad Aut\u00f3noma de Tlaxcala\u2010CINVESTAV Tlaxcala M\u00e9xico"},{"name":"\u00c1rea de Neurociencias Departamento de Biolog\u00eda de la Reproducci\u00f3n Universidad Aut\u00f3noma Metropolitana Iztapalapa M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,11,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0018-506X(71)90001-8"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-30405-2_3"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(68)90027-9"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(68)90090-5"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2013.04.012"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0018-506X(75)90013-6"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jne.12103"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0018-506X(77)90069-1"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1037\/bne0000103"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2016.08.010"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2017.12.005"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1962.203.3.493"},{"key":"e_1_2_7_14_1","first-page":"757461","article-title":"Estrogen deficiency and the origin of obesity during menopause","volume":"2014","author":"Lizcano F","year":"2014","journal-title":"Biomed Res"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2012-1055"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2014.04.018"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-106-2-504"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/1467-9450.00339"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1286.015"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2005.02.068"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2016.03.010"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-009-0017-3"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00026.2006"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.59.2.632"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1159\/000338668"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-107-3-774"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0083-6729(05)71005-0"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1159\/000338400"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2009.01846.x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2018.01.010"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2014.09.008"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2013-1844"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199811000-00011"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00702-005-0371-8"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.6.8071"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-17-06672.1998"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2004.12.027"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.18.8517"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-2760(01)00377-0"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1989.tb09136.x"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)90092-P"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.138.9.5489"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/jne.12065"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.7.2008"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20162"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(81)90019-6"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/0018-506X(82)90011-3"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1986.tb28018.x"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1006\/hbeh.1997.1413"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2826.1999.00298.x"},{"key":"e_1_2_7_51_1","first-page":"589","article-title":"Effect of guanine derivatives on lordosis behavior in estrogen primed rats","volume":"31","author":"Fern\u00e1ndez\u2010Guasti A","year":"1983","journal-title":"Physiol Behav"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2013.05.014"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2005.08.011"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2008.01794.x"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2006.12.022"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.npep.2011.09.003"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2003.08.003"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2006.10.007"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2008.12.019"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2010.03.014"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-0823"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2012.09.004"},{"key":"e_1_2_7_63_1","volume-title":"The Rat Brain in Stereotaxic Coordinates","author":"Paxinos G","year":"2006"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1037\/h0032536"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.268.1.C171"},{"key":"e_1_2_7_66_1","volume-title":"Estad\u00edstica no param\u00e9trica: aplicada a las ciencias de la conducta","author":"Siegel S","year":"1995"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijnurstu.2012.01.015"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.2.695"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.12.193"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.17.7686"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10194-017-0760-z"},{"key":"e_1_2_7_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(01)03043-8"},{"key":"e_1_2_7_73_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0404.2004.00306.x"},{"key":"e_1_2_7_74_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-05-01848.1998"},{"key":"e_1_2_7_75_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.17.7686"},{"key":"e_1_2_7_76_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1038-10.2010"},{"key":"e_1_2_7_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(73)90219-9"},{"key":"e_1_2_7_78_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.66.032802.154945"},{"key":"e_1_2_7_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2012.08.001"},{"key":"e_1_2_7_80_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend.14.10.0532"},{"key":"e_1_2_7_81_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.13121"},{"key":"e_1_2_7_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2016.06.014"},{"key":"e_1_2_7_83_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M212312200"},{"key":"e_1_2_7_84_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.11.5930"},{"key":"e_1_2_7_85_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.18.11902"},{"key":"e_1_2_7_86_1","doi-asserted-by":"publisher","DOI":"10.1038\/35035131"},{"key":"e_1_2_7_87_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.51.4.605"},{"key":"e_1_2_7_88_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(94)91295-5"},{"key":"e_1_2_7_89_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-16-07191.1999"}],"container-title":["Journal of Neuroendocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jne.12809","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jne.12809","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jne.12809","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T10:38:21Z","timestamp":1694169501000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jne.12809"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,28]]},"references-count":88,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10.1111\/jne.12809"],"URL":"https:\/\/doi.org\/10.1111\/jne.12809","archive":["Portico"],"relation":{},"ISSN":["0953-8194","1365-2826"],"issn-type":[{"value":"0953-8194","type":"print"},{"value":"1365-2826","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,28]]},"assertion":[{"value":"2019-05-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12809"}}