{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T03:37:51Z","timestamp":1764905871467,"version":"3.41.2"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,2,11]],"date-time":"2020-02-11T00:00:00Z","timestamp":1581379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A monoclonal antibody against oxytocin was generated in 7a5 hybridoma cells derived from myeloma cells and lymphocytes from the spleen of mice immunised with a synthetic oxytocin peptide. The 7a5 monoclonal antibody bound with oxytocin in enzyme\u2010linked immunosorbent assays. 7a5 cell growth medium was diluted up to 5000\u2010fold and used for immunohistochemistry. First, to test the specificity of the 7a5 antibody against oxytocin, we stained brain tissues of oxytocin knockout mice, comprising mice in which the first exon of the oxytocin\u2010neurophysin gene is deleted. No 7a5 immunoreactivity was detected in the paraventricular nucleus (PVN) of the hypothalamus of oxytocin knockout mice; however, this area was strongly stained with the anti\u2010vasopressin polyclonal antibody, HM07. Tissue preparations of the wild\u2010type mouse PVN and supraoptic nucleus (SON) displayed 7a5 immunoreactivity that was indistinguishable from the staining produced with an anti\u2010oxytocin polyclonal antibody, HM06. The immunoreactivity of HM06 in the PVN was similar to that of an anti\u2010oxytocin monoclonal antibody, PS38. We then examined the cross\u2010reactivity of 7a5 with arginine vasopressin. The majority of cell soma and processes stained by 7a5 were not co\u2010stained with the vasopressin antibody in SON and PVN regions. Furthermore, the suprachiasmatic nucleus was stained by the vasopressin antibody but not by 7a5. These results demonstrate that 7a5 is a new anti\u2010oxytocin monoclonal antibody recognising oxytocin and not vasopressin; therefore, 7a5 can be used to investigate the role of oxytocin in the brain.<\/jats:p>","DOI":"10.1111\/jne.12815","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T17:06:52Z","timestamp":1574788012000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A monoclonal antibody raised against a synthetic oxytocin peptide stains mouse hypothalamic neurones"],"prefix":"10.1111","volume":"32","author":[{"given":"Yuji","family":"Kogami","sequence":"first","affiliation":[{"name":"SkySea Pharmaceutical Inc. 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