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We have previously shown that Creb3l1 serves an important role in body fluid homeostasis through its transcriptional control of the gene coding for antidiuretic hormone arginine vasopressin in the neuropeptide\u2010rich magnocellular neurones of the supraoptic nucleus. In response to osmotic stimuli such as dehydration, vasopressin magnocellular neurones undergo remarkable transcriptome changes, including increased Creb3l1 expression, to ensure that the supply of vasopressin meets demand. To determine where else Creb3l1 fits into the secretory cell supply chain, we performed RNA\u2010sequencing of\n                    <jats:italic>Creb3l1<\/jats:italic>\n                    knockdown anterior pituitary mouse corticotroph cell line AtT20. The target chosen for further investigation was\n                    <jats:italic>Pcsk1<\/jats:italic>\n                    , which encodes proprotein convertase enzyme 1 (PC1\/3). PC1\/3 is crucial for processing of neuropeptides and peptide hormones such as pro\u2010opiomelanocortin (POMC), proinsulin, proglucagon, vasopressin and oxytocin. Viral manipulations in supraoptic nuclei by over\u2010expression of\n                    <jats:italic>Creb3l1<\/jats:italic>\n                    increased\n                    <jats:italic>Pcsk1<\/jats:italic>\n                    , whereas\n                    <jats:italic>Creb3l1<\/jats:italic>\n                    knockdown decreased\n                    <jats:italic>Pcsk1<\/jats:italic>\n                    expression. In vitro promoter activity and binding studies showed that Creb3l1 was a transcription factor of the\n                    <jats:italic>Pcsk1<\/jats:italic>\n                    gene binding directly to a G\u2010box motif in the promoter. In the dehydrated rat anterior pituitary,\n                    <jats:italic>Creb3l1<\/jats:italic>\n                    and\n                    <jats:italic>Pcsk1<\/jats:italic>\n                    expression decreased in parallel compared to control, supporting our findings from manipulations in AtT20 cells and the supraoptic nucleus. No relationship was observed between\n                    <jats:italic>Creb3l1<\/jats:italic>\n                    and\n                    <jats:italic>Pcsk1<\/jats:italic>\n                    expression in the neurointermediate lobe of the pituitary, indicating a different mechanism of PC1\/3 synthesis by these POMC\u2010synthesising cells. Therefore, Creb3l1, by regulating the expression of\n                    <jats:italic>Pcsk1<\/jats:italic>\n                    , does not control the processing of POMC peptides in the intermediate lobe.\n                  <\/jats:p>","DOI":"10.1111\/jne.12851","type":"journal-article","created":{"date-parts":[[2020,4,12]],"date-time":"2020-04-12T01:19:44Z","timestamp":1586654384000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Transcription factor Creb3l1 regulates the synthesis of prohormone convertase enzyme PC1\/3 in endocrine cells"],"prefix":"10.1111","volume":"32","author":[{"given":"Mingkwan","family":"Greenwood","sequence":"first","affiliation":[{"name":"Translational Health Sciences Bristol Medical School University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Paterson","sequence":"additional","affiliation":[{"name":"Translational Health Sciences Bristol Medical School University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parveen Akhter","family":"Rahman","sequence":"additional","affiliation":[{"name":"Translational Health Sciences Bristol Medical School University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin Thomas","family":"Gillard","sequence":"additional","affiliation":[{"name":"Translational Health Sciences Bristol Medical School University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sydney","family":"Langley","sequence":"additional","affiliation":[{"name":"Translational Health Sciences Bristol Medical School University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasumasa","family":"Iwasaki","sequence":"additional","affiliation":[{"name":"Health Care Center Kochi University Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Murphy","sequence":"additional","affiliation":[{"name":"Translational Health Sciences Bristol Medical School University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0565-2748","authenticated-orcid":false,"given":"Michael Paul","family":"Greenwood","sequence":"additional","affiliation":[{"name":"Translational Health Sciences Bristol Medical School University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.433"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvr041"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.211102"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2019.00123"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054060"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566524016666160225153659"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0108528"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13041-015-0159-1"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2005.03596.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)00253-X"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2010.02093.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4343-13.2014"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201004062"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1530\/JME-15-0323"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0124956"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2017.00413"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.6.3.485"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-02-00563.1997"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215540205000704"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.13-03-01258.1993"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.59.261"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2015-1117"},{"key":"e_1_2_7_24_1","first-page":"e3348","article-title":"Production and titering of recombinant adeno\u2010associated viral vectors","volume":"57","author":"McClure C","year":"2011","journal-title":"J Vis Exp"},{"key":"e_1_2_7_25_1","unstructured":"AndrewsS.FastQC: a quality control tool for high throughput sequence data.2010.https:\/\/wwwbioinformaticsbabrahamacuk\/projects\/fastqc\/. 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