{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:44:03Z","timestamp":1790441043602,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,4,29]],"date-time":"2020-04-29T00:00:00Z","timestamp":1588118400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["AG059779"],"award-info":[{"award-number":["AG059779"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Growth hormone (GH) excess in bovine (b)GH transgenic mice has been shown to alter white adipose tissue (WAT) immune cell populations. The present study aimed to evaluate the effects of GH resistance on WAT immune cell populations using GH receptor knockout (GHR<jats:sup>\u2212\/\u2212<\/jats:sup>) mice. Eight\u2010 and 24\u2010month\u2010old, male GHR<jats:sup>\u2212\/\u2212<\/jats:sup> and wild\u2010type mice were used. Body composition and tissue weights were determined, and systemic inflammation was assessed by measuring serum cytokine levels. The stromal vascular fraction (SVF) was isolated from three distinct WAT depots, and immune cell populations were quantified using flow cytometry. GHR<jats:sup>\u2212\/\u2212<\/jats:sup> mice at both ages had decreased body weight but were obese. Although no significant changes were observed in serum levels of the measured cytokines, SVF cell alterations were seen and differed from depot to depot. Total SVF cells were decreased in epidydimal (Epi) depots, whereas SVF cells per gram adipose tissue weight were increased in mesenteric (Mes) depots of GHR<jats:sup>\u2212\/\u2212<\/jats:sup> mice relative to controls. T cells and T helper cells were increased in Mes at 8\u00a0months old, whereas cytotoxic T cells were decreased in subcutaneous\u00a0(SubQ) at 24\u00a0months old. Other cells were unchanged at both ages measured. The present study demonstrates that removal of GH action results in modest and depot\u2010specific changes to several immune cell populations in WAT of intra\u2010abdominal depots (Epi and Mes), which are somewhat surprising results\u00a0because the SubQ has the largest change in size, whereas the Mes has no size change. Taken together with previous results from bovine GH transgenic mice, these data suggest that GH induces changes in the immune cell population of WAT in a depot\u2010specific manner. Notably, GHR<jats:sup>\u2212\/\u2212<\/jats:sup> mice appear to be protected from age\u2010related WAT inflammation and immune cell infiltration despite obesity.<\/jats:p>","DOI":"10.1111\/jne.12854","type":"journal-article","created":{"date-parts":[[2020,4,12]],"date-time":"2020-04-12T20:47:18Z","timestamp":1586724438000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["GHR<sup>\u2212\/\u2212<\/sup> Mice are protected from obesity\u2010related white adipose tissue inflammation"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1770-1391","authenticated-orcid":false,"given":"Jonathan A.","family":"Young","sequence":"first","affiliation":[{"name":"Edison Biotechnology Institute Ohio University Athens OH USA"},{"name":"Department of Biomedical Sciences Heritage College of Osteopathic Medicine Ohio University Athens OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brooke E.","family":"Henry","sequence":"additional","affiliation":[{"name":"Edison Biotechnology Institute Ohio University Athens OH USA"},{"name":"School of Applied Health Sciences and Wellness College of Health Sciences and Professions Ohio University Athens OH USA"},{"name":"The Diabetes Institute at Ohio University Ohio University Athens OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"Benencia","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Heritage College of Osteopathic Medicine Ohio University Athens OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Bell","sequence":"additional","affiliation":[{"name":"The Diabetes Institute at Ohio University Ohio University Athens OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward O.","family":"List","sequence":"additional","affiliation":[{"name":"Edison Biotechnology Institute Ohio University Athens OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John J.","family":"Kopchick","sequence":"additional","affiliation":[{"name":"Edison Biotechnology Institute Ohio University Athens OH USA"},{"name":"Department of Biomedical Sciences Heritage College of Osteopathic Medicine Ohio University Athens OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darlene E.","family":"Berryman","sequence":"additional","affiliation":[{"name":"Edison Biotechnology Institute Ohio University Athens OH USA"},{"name":"Department of Biomedical Sciences Heritage College of Osteopathic Medicine Ohio University Athens OH USA"},{"name":"The Diabetes Institute at Ohio University Ohio University Athens OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1090470110"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2007.04.004"},{"key":"e_1_2_6_4_1","first-page":"78210","article-title":"Can growth hormone (GH) accelerate aging? 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