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It has been hypothesised that exposure to endogenous ovarian hormones during adolescence enhances the susceptibility of females to mood disorders from this stage of life onwards. However, experimental evidence in favour of this hypothesis is lacking. In the present study, we examined the long\u2010term effects of suppressing adolescent gonadal hormone levels in a group of female Lister\u2010hooded rats via administration of a gonadotrophin\u2010releasing hormone antagonist (Antide; administered on postnatal day [PND] 28 and 42) compared to control females and males (n\u00a0=\u00a014 per group). We predicted that, in adulthood, Antide\u2010treated female rats would exhibit more male\u2010like behaviour than control females in novel environments (elevated\u2010plus maze, open field and light\u2010dark box), in response to novel objects and novel social partners, and in an acoustic startle task. Progesterone and luteinising hormone assays (which were conducted on blood samples collected on PND 55\/56 and 69\/70) confirmed that the hypothalamic\u2010pituitary\u2010gonadal axis was temporarily suppressed by Antide treatment. In addition, Antide\u2010treated females were found to exhibit a modest pubertal delay, as measured by vaginal opening, which was comparable in length to the pubertal delay that has been induced by adolescent exposure to alcohol or stress in previous studies of female rats. However, Antide\u2010treated females did not substantially differ from control females on any of the behavioural tests, despite the evidence for predicted sex differences in some measures. Following the acoustic startle response task, all subjects were culled and perfused, and c\u2010Fos staining was conducted in the medial and basolateral amygdala, with the results showing no significant differences in cell counts between the groups. These findings suggest that ovarian hormone exposure during adolescence does not have long\u2010term effects on anxiety\u2010related responses in female rats.<\/jats:p>","DOI":"10.1111\/jne.12897","type":"journal-article","created":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T15:11:44Z","timestamp":1596553904000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Suppression of ovarian hormones in adolescent rats has no effect on anxiety\u2010like behaviour or <i>c\u2010fos<\/i> activation in the amygdala"],"prefix":"10.1111","volume":"32","author":[{"given":"Amy R.","family":"Hodgson","sequence":"first","affiliation":[{"name":"School of Psychology &amp; Neuroscience University of St Andrews  St Andrews UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Richmond","sequence":"additional","affiliation":[{"name":"School of Psychology &amp; Neuroscience University of St Andrews  St 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