{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T05:20:52Z","timestamp":1780550452118,"version":"3.54.1"},"reference-count":71,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,9,9]],"date-time":"2020-09-09T00:00:00Z","timestamp":1599609600000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Feeding is a complex behaviour entailing elaborate interactions between forebrain, hypothalamic and brainstem neuronal circuits via multiple orexigenic and anorexigenic neuropeptides. Nucleobindin\u20102 (NUCB2)\/nesfatin\u20101 is a negative regulator of food intake and body weight with a widespread distribution in rodent brainstem nuclei. However, its localisation pattern in the human brainstem is unknown. The present study aimed to explore NUCB2\/nesfatin\u20101 immunoexpression in human brainstem nuclei and its possible correlation with body weight. Sections of human brainstem from 20 autopsy cases (13 males, seven females; eight normal weight, six overweight, six obese) were examined using immunohistochemistry and double immunofluorescence labelling. Strong immunoreactivity for NUCB2\/nesfatin\u20101 was displayed in various brainstem areas, including the locus coeruleus, medial and lateral parabrachial nuclei, pontine nuclei, raphe nuclei, nucleus of the solitary tract, dorsal motor nucleus of vagus (10N), area postrema, hypoglossal nucleus, reticular formation, inferior olive, cuneate nucleus, and spinal trigeminal nucleus. NUCB2\/nesfatin\u20101 was shown to extensively colocalise with neuropeptide Y and cocaine\u2010 and amphetamine\u2010regulated transcript in the locus coeruleus, dorsal raphe nucleus and solitary tract. Interestingly, in the examined cases, NUCB2\/nesfatin\u20101 protein expression was lower in obese than normal weight subjects in the solitary tract (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.020). The findings of the present study provide neuroanatomical support for a role for NUCB2\/nesfatin\u20101 in feeding behaviour and energy balance. The widespread distribution of NUCB2\/nesfatin\u20101 in the human brainstem nuclei may be indicative of its pleiotropic effects on autonomic, neuroendocrine and behavioural processes. In the solitary tract, a key integrator of energy status, altered neurochemistry may contribute to obesity. Further research is necessary to decipher human brainstem energy homeostasis circuitry, which, despite its importance, remains inadequately characterised.\n                  <\/jats:p>","DOI":"10.1111\/jne.12899","type":"journal-article","created":{"date-parts":[[2020,8,8]],"date-time":"2020-08-08T03:43:17Z","timestamp":1596858197000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Nucleobindin 2\/nesfatin\u20101 expression and colocalisation with neuropeptide Y and cocaine\u2010 and amphetamine\u2010regulated transcript in the human brainstem"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9609-6710","authenticated-orcid":false,"given":"Aristea","family":"Psilopanagioti","sequence":"first","affiliation":[{"name":"Department of Anatomy\u2010Histology\u2010Embryology School of Medicine University of Patras  Patras Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Makrygianni","sequence":"additional","affiliation":[{"name":"Department of Anatomy\u2010Histology\u2010Embryology School of Medicine University of Patras  Patras Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Nikou","sequence":"additional","affiliation":[{"name":"Department of Anatomy\u2010Histology\u2010Embryology School of Medicine University of Patras  Patras Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souzana","family":"Logotheti","sequence":"additional","affiliation":[{"name":"Department of Pathology School of Medicine University of Patras  Patras Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Papadaki","sequence":"additional","affiliation":[{"name":"Department of Anatomy\u2010Histology\u2010Embryology School of Medicine University of Patras  Patras Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0149-7634(02)00014-3"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/frne.2001.0224"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/0-306-48643-1_9"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/35007534"},{"key":"e_1_2_8_6_1","volume-title":"Human Brainstem. 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