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The present study aimed to determine whether nesfatin\u20101 plays a role in fish somatic growth by modulating the growth hormone (GH)\/insulin\u2010like growth factor (IGF) axis, using a representative teleost model, the goldfish (<jats:italic>Carassius auratus<\/jats:italic>). The results demonstrated that a single i.p. injection of synthetic goldfish nesfatin\u20101 significantly decreased the expression of hypothalamic <jats:italic>pacap<\/jats:italic> (approximately 90%) and pituitary <jats:italic>Gh<\/jats:italic> (approximately 90%) mRNAs at 15\u00a0minutes post\u2010injection. Serum GH levels were also reduced as a result of nesfatin\u20101 administration, by approximately 45% and 55% at 15 and 30\u00a0minutes post\u2010injection, respectively. Likewise, in vitro treatment of goldfish dispersed pituitary cells with nesfatin\u20101 reduced Gh secretion, suggesting that nesfatin\u20101 acts directly on pituitary somatotrophs to inhibit Gh release. Exposure of cultured liver fragments to nesfatin\u20101 (0.1, 1 and 10\u00a0nmol\u00a0L<jats:sup>\u20101<\/jats:sup>) led to a significant reduction in <jats:italic>igf\u20101<\/jats:italic> mRNA at 120\u00a0minutes and of <jats:italic>igf\u2010II<\/jats:italic> mRNA at 30 and 60\u00a0minutes post\u2010incubation. Collectively, these results indicate a suppressive role for nesfatin\u20101 on the goldfish GH\/IGF axis. Immunohistochemical studies demonstrated that NUCB2\/nesfatin\u20101\u2010like immunoreactivity, although present in the goldfish pituitary, is not colocalised with GH in goldfish somatotrophs. Thus, nesfatin\u20101 does not appear to act in an autocrine manner to regulate GH secretion. Taken together, this research found that the pituitary gland is an important source of endogenous NUCB2\/nesfatin\u20101 and also that nesfatin\u20101 directly suppresses the Gh\/IGF axis in goldfish.<\/jats:p>","DOI":"10.1111\/jne.13010","type":"journal-article","created":{"date-parts":[[2021,6,26]],"date-time":"2021-06-26T13:01:56Z","timestamp":1624712516000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Nesfatin\u20101 is an inhibitor of the growth hormone\u2010insulin\u2010like growth factor axis in goldfish (<i>Carassius auratus<\/i>)"],"prefix":"10.1111","volume":"33","author":[{"given":"Ayel\u00e9n M.","family":"Blanco","sequence":"first","affiliation":[{"name":"Laboratory of Integrative Neuroendocrinology Department of Veterinary Biomedical Sciences Western College of Veterinary Medicine, University of Saskatchewan Saskatoon SK Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua 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