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Pituitary tumors were induced in female F344 rats with estradiol benzoate for 5, 10, 20 and 30\u00a0days. Cells positive for GFRa2, Sox2, Sox9, Nestin, CD133 and CD44 were identified in the marginal zone and in the adenoparenchyma in both control and 30D groups, with predominant adenoparenchyma localization of GRFa2 and SOX9 found in tumoral pituitaries. GFRa2, Nestin, CD133 and CD44 were upregulated at the initial stages of tumor growth, whereas Sox9 significantly decreased at 5D, with Sox2 remaining invariable during the hyperplasic\/adenomatous development. In addition, isolated pituispheres from normal and tumoral pituitary glands enriched in SC\/TSC were characterized. Pituispheres from the 30D glands were positive for the above\u2010mentioned markers and showed a significant increase in the proliferation. In conclusion, our data revealed pituitary SC pool fluctuations during hyperplastic\/adenomatous development, with differential localization of the SC\/TSC niche in this process. These findings may help to provide a better understanding of these cell populations, which is crucial for achieving advancements in the field of pituitary tumor biology.<\/jats:p>","DOI":"10.1111\/jne.13051","type":"journal-article","created":{"date-parts":[[2021,10,9]],"date-time":"2021-10-09T12:21:50Z","timestamp":1633782110000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Changes of stem cell niche during experimental pituitary tumor development"],"prefix":"10.1111","volume":"33","author":[{"given":"Carolina Beatriz","family":"Guido","sequence":"first","affiliation":[{"name":"Centro de Microscop\u00eda Electr\u00f3nica Facultad de Ciencias M\u00e9dicas Universidad Nacional de C\u00f3rdoba C\u00f3rdoba Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Instituto de Investigaciones en Ciencias de la Salud C\u00f3rdoba 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