{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T07:51:23Z","timestamp":1776153083157,"version":"3.50.1"},"reference-count":69,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T00:00:00Z","timestamp":1635292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The neural cell adhesion molecule (NCAM) has previously been studied in pituitary neuroendocrine tumours (PitNETs), but its role in tumour biology and aggressiveness remains controversial, and its relationship with the tumour microenvironment remains unknown. We aimed to characterise NCAM expression in PitNETs, to correlate this with clinico\u2010pathological features, and to assess the role of various microenvironment components on NCAM expression. NCAM and immune cells were investigated by immunohistochemistry in 16\u00a0human non\u2010functioning\u2010PitNETs (NF\u2010PitNETs) and eight somatotrophinomas, including macrophages (CD68, CD163, HLA\u2010DR), cytotoxic (CD8) and T helper (CD4) lymphocytes, regulatory T cells (FOXP3), B cells (CD20), and neutrophils (neutrophil elastase). Five normal pituitaries were included for comparison. The cytokine secretome from these PitNETs and from PitNET\u2010derived tumour\u2010associated fibroblasts (TAFs) were assessed on culture supernatants using a multiplex immunoassay panel. There were no significant NCAM expression differences between PitNETs and normal pituitary, and no difference between types of pituitary tumours (NF\u2010PitNETs vs. somatotrophinomas). There was no association between NCAM expression and different clinico\u2010pathological features, including cavernous sinus invasion and Ki\u201067, nor with serum hormone levels. NCAM immunoreactivity correlated negatively with PitNET\u2010derived CXCL10 (rho\u00a0=\u00a0\u22120.417;\n                    <jats:italic>p\u00a0<\/jats:italic>\n                    =\u00a0.042) and CX3CL1 (rho\u00a0=\u00a0\u22120.423;\n                    <jats:italic>p\u00a0<\/jats:italic>\n                    =\u00a0.040) levels. NCAM immunoreactivity was negatively correlated with TAF\u2010derived fibroblast growth factor (FGF)\u20102 (rho\u00a0=\u00a0\u22120.632;\n                    <jats:italic>p\u00a0<\/jats:italic>\n                    =\u00a0.009), but not with other TAF\u2010derived cytokines. Within the PitNET cohort, there were no correlations between NCAM immunoreactivity and immune infiltrates or ratios, although, within NF\u2010PitNETs, NCAM expression was higher in tumours with more FOXP3+ cells. NCAM expression does not differ between PitNETs and normal pituitary, and does not appear to relate to tumour invasiveness or proliferation. However, our data suggest a possible role for cytokines in the modulation of NCAM expression in PitNETs, particularly CXCL10, CX3CL1 and FGF\u20102, but not for immune cell infiltrates.\n                  <\/jats:p>","DOI":"10.1111\/jne.13052","type":"journal-article","created":{"date-parts":[[2021,10,9]],"date-time":"2021-10-09T08:10:16Z","timestamp":1633767016000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The expression of neural cell adhesion molecule and the microenvironment of pituitary neuroendocrine tumours"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4959-5725","authenticated-orcid":false,"given":"Pedro","family":"Marques","sequence":"first","affiliation":[{"name":"Centre for Endocrinology, Barts and the London School of Medicine and Dentistry William Harvey Research Institute Queen Mary University of London London UK"},{"name":"Department of Endocrinology Hospital de Santa Maria Centro Hospitalar Universit\u00e1rio Lisboa Norte Lisbon Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sayka","family":"Barry","sequence":"additional","affiliation":[{"name":"Centre for Endocrinology, Barts and the London School of Medicine and Dentistry William Harvey Research Institute Queen Mary University of London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eivind","family":"Carlsen","sequence":"additional","affiliation":[{"name":"Department of Pathology STHF Skien Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Collier","sequence":"additional","affiliation":[{"name":"Centre for Endocrinology, Barts and the London School of Medicine and Dentistry William Harvey Research Institute Queen Mary University of London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy","family":"Ronaldson","sequence":"additional","affiliation":[{"name":"Centre for Endocrinology, Barts and the London School of Medicine and Dentistry William Harvey Research Institute Queen Mary University of London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan","family":"Grieve","sequence":"additional","affiliation":[{"name":"The National Hospital for Neurology and Neurosurgery UCLH NHS Trust London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil","family":"Dorward","sequence":"additional","affiliation":[{"name":"The National Hospital for Neurology and Neurosurgery UCLH NHS Trust London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nigel","family":"Mendoza","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Charing Cross Hospital Imperial College London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramesh","family":"Nair","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Charing Cross Hospital Imperial College London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samiul","family":"Muquit","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Derriford Hospital Plymouth UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1176-6186","authenticated-orcid":false,"given":"Ashley B.","family":"Grossman","sequence":"additional","affiliation":[{"name":"Centre for Endocrinology, Barts and the London School of Medicine and Dentistry William Harvey Research Institute Queen Mary University of London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4101-9432","authenticated-orcid":false,"given":"M\u00e1rta","family":"Korbonits","sequence":"additional","affiliation":[{"name":"Centre for Endocrinology, Barts and the London School of Medicine and Dentistry William Harvey Research Institute Queen Mary University of London London 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