{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T02:51:22Z","timestamp":1783651882761,"version":"3.55.0"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,1,25]],"date-time":"2022-01-25T00:00:00Z","timestamp":1643068800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["289568"],"award-info":[{"award-number":["289568"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["588869"],"award-info":[{"award-number":["588869"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005739","name":"Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"publisher","award":["405PC"],"award-info":[{"award-number":["405PC"]}],"id":[{"id":"10.13039\/501100005739","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Excessive vasopermeability and angiogenesis compromise vision in diabetic macular oedema (DME) and diabetic retinopathy (DR). Vasoinhibin is a fragment of the hormone prolactin (PRL) that inhibits diabetes\u2010induced retinal hypervasopermeability and ischaemia\u2010induced retinal angiogenesis in rodents. Hyperprolactinaemia generated by the dopamine D2 receptor antagonist, levosulpiride, is associated with higher levels of vasoinhibin in the vitreous of patients with DR, implying a beneficial outcome due to vasoinhibin\u2010mediated inhibition of retinal vascular alterations. Here, we tested whether hyperprolactinaemia induced by racemic sulpiride increases intraocular vasoinhibin levels and inhibits retinal hypervasopermeability in diabetic rats. Diabetes was generated with streptozotocin and, 4\u00a0weeks later, rats were treated for 2\u00a0weeks with sulpiride or osmotic minipumps delivering PRL. ELISA, Western blot, and Evans blue assay were used to evaluate serum PRL, retinal vasoinhibin, and retinal vasopermeability, respectively. Hyperprolactinaemia in response to sulpiride or exogenous PRL was associated with increased levels of vasoinhibin in the retina and reduced retinal hypervasopermeability. Furthermore, sulpiride decreased retinal haemorrhages in response to the intravitreal administration of vascular endothelial growth factor (VEGF). Neither sulpiride nor exogenous PRL modified blood glucose levels or bodyweight. We conclude that sulpiride\u2010induced hyperprolactinaemia inhibits the diabetes\u2010 and VEGF\u2010mediated increase in retinal vasopermeability by promoting the intraocular conversion of endogenous PRL to vasoinhibin. These findings support the therapeutic potential of sulpiride and its levorotatory enantiomer, levosulpiride, against DME and DR.<\/jats:p>","DOI":"10.1111\/jne.13091","type":"journal-article","created":{"date-parts":[[2022,1,14]],"date-time":"2022-01-14T12:35:26Z","timestamp":1642163726000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Sulpiride\u2010induced hyperprolactinaemia increases retinal vasoinhibin and protects against diabetic retinopathy in rats"],"prefix":"10.1111","volume":"34","author":[{"given":"Elva","family":"Ad\u00e1n\u2010Castro","sequence":"first","affiliation":[{"name":"Instituto de Neurobiolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM)  Quer\u00e9taro M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourdes","family":"Siqueiros\u2010M\u00e1rquez","sequence":"additional","affiliation":[{"name":"Instituto de Neurobiolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM)  Quer\u00e9taro M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Ram\u00edrez\u2010Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Instituto de Neurobiolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM)  Quer\u00e9taro M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nundehui","family":"D\u00edaz\u2010Lezama","sequence":"additional","affiliation":[{"name":"Instituto de Neurobiolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM)  Quer\u00e9taro M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xarubet","family":"Ru\u00edz\u2010Herrera","sequence":"additional","affiliation":[{"name":"Instituto de Neurobiolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM)  Quer\u00e9taro M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Freinet","family":"N\u00fa\u00f1ez","sequence":"additional","affiliation":[{"name":"Instituto de Neurobiolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM)  Quer\u00e9taro M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos D.","family":"N\u00fa\u00f1ez\u2010Amaro","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Naturales Universidad Aut\u00f3noma de Quer\u00e9taro  Quer\u00e9taro M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma. Ludivina","family":"Robles\u2010Osorio","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Naturales Universidad Aut\u00f3noma de Quer\u00e9taro  Quer\u00e9taro M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Bertsch","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry, Laboratory Medicine and Transfusion Medicine Nuremberg General Hospital &amp; Paracelsus Medical University  Nuremberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob","family":"Triebel","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry, Laboratory Medicine and Transfusion Medicine Nuremberg General Hospital &amp; Paracelsus Medical University  Nuremberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gonzalo","family":"Mart\u00ednez de la Escalera","sequence":"additional","affiliation":[{"name":"Instituto de Neurobiolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM)  Quer\u00e9taro M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7670-6718","authenticated-orcid":false,"given":"Carmen","family":"Clapp","sequence":"additional","affiliation":[{"name":"Instituto de Neurobiolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM)  Quer\u00e9taro M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,1,25]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"1","volume-title":"IDF Diabetes Atlas","author":"International Diabetes Federation","year":"2019"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc11-1909"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000438970"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11892-019-1188-4"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2006.08.002"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00024.2009"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-12114-7_4"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00256.2015"},{"key":"e_1_2_9_10_1","first-page":"2498","article-title":"Inhibition of rat corneal angiogenesis by 16\u2010kDa prolactin and by endogenous prolactin\u2010like molecules","volume":"40","author":"Due\u00f1as Z","year":"1999","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-0173"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/pr.2016.241"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jne.12858"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1530\/EJE-09-0130"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.03-1001"},{"key":"e_1_2_9_16_1","first-page":"2291","article-title":"Vasoinhibins prevent retinal vasopermeability associated with diabetic retinopathy in rats via protein phosphatase 2A\u2010dependent eNOS inactivation","volume":"118","author":"Garc\u00eda C","year":"2008","journal-title":"J Clin Invest"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.11-8190"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2015.135"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2018.00242"},{"key":"e_1_2_9_20_1","first-page":"1034","article-title":"Role of levosulpiride in the management of functional dyspepsia","volume":"2","author":"Ratnani I","year":"2015","journal-title":"J Fam Med"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijem.IJEM_555_16"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1167\/tvst.9.9.27"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-121-6-2055"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02913315"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2014-1571"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-51-5-1058"},{"key":"e_1_2_9_27_1","first-page":"E520","article-title":"Epitope mapping of monoclonal antibodies to bovine prolactin","volume":"263","author":"Scammell JG","year":"1992","journal-title":"Am J Physiol"},{"key":"e_1_2_9_28_1","first-page":"789","article-title":"Sensitive blood\u2010retinal barrier breakdown quantitation using Evans blue","volume":"42","author":"Xu Q","year":"2001","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00327.2014"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1005073"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02887"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1210\/endocr\/bqab177"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1177\/2042018813512360"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.03-1346"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1159\/000265174"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.2337\/db10-0873"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-003-1088-6"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(14)75794-0"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(01)01269-7"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-0647"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(02)03630-X"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704956"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph4071052"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2006.01998.x"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/87895"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00272.2004"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(02)01816-2"},{"key":"e_1_2_9_48_1","first-page":"1989","article-title":"Gastrokinetic effects of levosulpiride in dyspeptic patients with diabetic gastroparesis","volume":"90","author":"Mansi C","year":"1995","journal-title":"Am J Gastroenterol"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2020.01.004"}],"container-title":["Journal of Neuroendocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jne.13091","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jne.13091","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jne.13091","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T03:44:52Z","timestamp":1692848692000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jne.13091"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,25]]},"references-count":48,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["10.1111\/jne.13091"],"URL":"https:\/\/doi.org\/10.1111\/jne.13091","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/JNE.13091\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNE.13091\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNE.13091\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNE.13091\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNE.13091\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNE.13091\/v2\/response1","asserted-by":"object"}]},"ISSN":["0953-8194","1365-2826"],"issn-type":[{"value":"0953-8194","type":"print"},{"value":"1365-2826","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,25]]},"assertion":[{"value":"2021-10-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13091"}}