{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T03:25:58Z","timestamp":1790565958060,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,8,19]],"date-time":"2022-08-19T00:00:00Z","timestamp":1660867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009193","name":"Marsden Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009193","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Agouti\u2010related peptide (AgRP) neurons are thought to indirectly regulate the activity of hypothalamic gonadotrophin\u2010releasing hormone neurons which control fertility. AgRP neurons also drive caloric intake and are modulated by metabolically\u2010relevant hormones, providing a link to the hypothalamic\u2013pituitary\u2010gonadal axis. In mice expressing Cre\u2010dependant designer receptors (DREADDs) in AgRP neurons, we activated or silenced these neurons in vivo using the synthetic ligand clozapine\u2010N\u2010oxide (CNO) to observe the effect of AgRP neuron activity on timing of puberty. To validate these animals, we chronically treated both stimulatory (hM3Dq) and inhibitory (hM4Di) DREADD\u2009\u00d7\u2009AgRP\u2010Cre mice with CNO, observing a pronounced increase and decrease of food intake, respectively, consistent with the known orexigenic effects of these neurons. RNAscope was performed to visually confirm the activation of AgRP neurons. Puberty onset was assessed in males and females. There was no effect on preputial separation in males or vaginal opening and first oestrus in females after CNO treatment from day 26 to 30 to chronically modulate AgRP neurons. Next, to determine whether the delay in puberty onset occurring in response to neonatal underfeeding could be overcome by inhibiting AgRP neuronal activity, mice were raised in large (neonatally underfed) or normal litter sizes. The delay in puberty from underfeeding was completely reversed in CNO\u2010treated AgRP\u2010hM4Di male mice. These data highlight the inhibitory role of AgRP neurons to delay puberty onset when undernutrition occurs during the neonatal period, at least in male mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Trail registration number<\/jats:title>\n                    <jats:p>JNE\u201022\u20100081\u2010OA.R2<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jne.13190","type":"journal-article","created":{"date-parts":[[2022,7,31]],"date-time":"2022-07-31T05:09:45Z","timestamp":1659244185000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Agouti\u2010related peptide neuronal silencing overcomes delayed puberty in neonatally underfed male mice"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2181-0622","authenticated-orcid":false,"given":"Caroline","family":"Decourt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George A. 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P.","family":"Connolly","sequence":"additional","affiliation":[{"name":"Centre for Neuroendocrinology and Department of Anatomy University of Otago School of Biomedical Sciences  Dunedin New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Ancel","sequence":"additional","affiliation":[{"name":"Centre for Neuroendocrinology and Department of Anatomy University of Otago School of Biomedical Sciences  Dunedin New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan A.","family":"Inglis","sequence":"additional","affiliation":[{"name":"Centre for Neuroendocrinology and Department of Anatomy University of Otago School of Biomedical Sciences  Dunedin New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6747-8047","authenticated-orcid":false,"given":"Greg M.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Centre for Neuroendocrinology and Department 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