{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T21:28:27Z","timestamp":1773955707526,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T00:00:00Z","timestamp":1662508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Sex differences in emotional behaviors and affective disorders have been widely noted, of which sexually dimorphic secretion of gonadal steroid hormones such as estrogen is suspected to play a role. However, the underlying neural mechanisms remain poorly understood. We noted that the expression of estrogen receptor 2 (\n                    <jats:italic>Esr2<\/jats:italic>\n                    , or ER\u03b2), a key mediator of estrogen signaling in the brain, was enriched in the dorsal raphe nucleus (DRN), a region involved in emotion regulation. To investigate whether DRN\n                    <jats:italic>Esr2<\/jats:italic>\n                    expression confers sex\u2010specific susceptibility or vulnerability in emotional behaviors, we generated a conditional allele of\n                    <jats:italic>Esr2<\/jats:italic>\n                    that allowed for site\u2010specific deletion of\n                    <jats:italic>Esr2<\/jats:italic>\n                    in the DRN via local injection of Cre\u2010expressing viruses. DRN\u2010specific\n                    <jats:italic>Esr2<\/jats:italic>\n                    deletion mildly increased anxiety behaviors in females, as shown by decreased time spent in the center zone of an open field in knockout females. By contrast, DRN\n                    <jats:italic>Esr2<\/jats:italic>\n                    deletion had no effects on anxiety levels in males, as demonstrated by knockout males spending comparable time in the center zone of an open field and open arms of an elevated\u2010plus maze. Furthermore, in the tail suspension test, DRN\n                    <jats:italic>Esr2<\/jats:italic>\n                    deletion reduced immobility, a depression\u2010like behavior, in a male\u2010biased manner. Together, these results reveal sex\u2010specific functions of DRN\n                    <jats:italic>Esr2<\/jats:italic>\n                    in regulating emotional behaviors and suggest targeted manipulation of DRN\n                    <jats:italic>Esr2<\/jats:italic>\n                    signaling as a potential therapeutic strategy to treat sex\u2010biased affective disorders.\n                  <\/jats:p>","DOI":"10.1111\/jne.13195","type":"journal-article","created":{"date-parts":[[2022,8,20]],"date-time":"2022-08-20T02:55:57Z","timestamp":1660964157000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Sexually dimorphic effects of estrogen receptor 2 deletion in the dorsal raphe nucleus on emotional behaviors"],"prefix":"10.1111","volume":"35","author":[{"given":"Jing","family":"He","sequence":"first","affiliation":[{"name":"Department of Psychiatry, Tongji Hospital of Tongji University Tongji University 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