{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:51:49Z","timestamp":1772160709009,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,5,9]],"date-time":"2023-05-09T00:00:00Z","timestamp":1683590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["HI 2206\/2\u20101"],"award-info":[{"award-number":["HI 2206\/2\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["TRR 127"],"award-info":[{"award-number":["TRR 127"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Growth hormone receptor deficiency (GHRD) results in low serum insulin\u2010like growth factor 1 (IGF1) and high, but non\u2010functional serum growth hormone (GH) levels in human Laron syndrome (LS) patients and animal models. This study investigated the quantitative histomorphological and molecular alterations associated with GHRD. Pituitary glands from 6\u2009months old growth hormone receptor deficient (\n                    <jats:italic>GHR<\/jats:italic>\n                    \u2010KO) and control pigs were analyzed using a quantitative histomorphological approach in paraffin (9\n                    <jats:italic>GHR<\/jats:italic>\n                    \u2010KO [5 males, 4 females] vs. 11 controls [5 males, 6 females]), ultrathin sections tissue sections (3 male\n                    <jats:italic>GHR<\/jats:italic>\n                    \u2010KO vs. 3 male controls) and label\u2010free proteomics (4\n                    <jats:italic>GHR<\/jats:italic>\n                    \u2010KO vs. 4 control pigs [2 per sex]).\n                    <jats:italic>GHR<\/jats:italic>\n                    \u2010KO pigs displayed reduced body weights (60% reduction in comparison to controls;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.0001) and decreased pituitary volumes (54% reduction in comparison to controls;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.0001). The volume proportion of the adenohypophysis did not differ in\n                    <jats:italic>GHR<\/jats:italic>\n                    \u2010KO and control pituitaries (65% vs. 71%;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u2009.0506) and\n                    <jats:italic>GHR<\/jats:italic>\n                    \u2010KO adenohypophyses displayed a reduced absolute volume but an unaltered volume density of somatotrophs in comparison to controls (21% vs. 18%;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u2009.3164). In\n                    <jats:italic>GHR<\/jats:italic>\n                    \u2010KO pigs, somatotroph cells displayed a significantly reduced volume density of granules (23.5%) as compared to controls (67.7%;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.0001). Holistic proteome analysis of adenohypophysis samples identified 4660 proteins, of which 592 were differentially abundant between the\n                    <jats:italic>GHR<\/jats:italic>\n                    \u2010KO and control groups. In\n                    <jats:italic>GHR<\/jats:italic>\n                    \u2010KO samples, the abundance of somatotropin precursor was decreased, whereas increased abundances of proteins involved in protein production, transport and endoplasmic reticulum (ER) stress were revealed. Increased protein production and secretion as well as significantly reduced proportion of GH\u2010storing granules in somatotroph cells of the adenohypophysis without an increase in volume density of somatotroph cells in the adenohypophysis could explain elevated serum GH levels in\n                    <jats:italic>GHR<\/jats:italic>\n                    \u2010KO pigs.\n                  <\/jats:p>","DOI":"10.1111\/jne.13277","type":"journal-article","created":{"date-parts":[[2023,4,15]],"date-time":"2023-04-15T09:14:40Z","timestamp":1681550080000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Structural and proteomic repercussions of growth hormone receptor deficiency on the pituitary gland: Lessons from a translational pig model"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4558-0785","authenticated-orcid":false,"given":"Bachuki","family":"Shashikadze","sequence":"first","affiliation":[{"name":"Laboratory for Functional Genome Analysis (LAFUGA), Gene Center Ludwig\u2010Maximilians\u2010Universit\u00e4t M\u00fcnchen  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1265-6790","authenticated-orcid":false,"given":"Sophie","family":"Franzmeier","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Pathology at the Center for Clinical Veterinary Medicine, Ludwig\u2010Maximilians\u2010Universit\u00e4t M\u00fcnchen  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4019-9619","authenticated-orcid":false,"given":"Isabel","family":"Hofmann","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Pathology at the Center 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