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Astrocytes play an active role in regulating DVC function and, by extension, physiological parameters. DVC astrocytes in ex vivo slices respond to low tissue glucose. The response of neurons to low glucose is conditional on intact astrocyte signalling in slice preparations, suggesting astrocytes are primary sensors of glucose deprivation (glucoprivation). Based on these published findings we hypothesised that in vivo DVC astrocyte manipulation with chemogenetics would be sufficient to alter physiological responses that control blood glucose. We found that 2\u2010h after systemic 2\u2010DG\u2010induced glucoprivation there were no observable changes in morphology of glial fibrillary acidic protein (GFAP)\u2010immunoreactive DVC cells, specifically those in the nucleus of the solitary tract (NTS). Chemogenetic activation of DVC astrocytes was sufficient to suppress nocturnal food intake by reducing both meal size and meal number and this manipulation also suppressed 2\u2010DG\u2010induced glucoprivic food intake. Chemogenetic activation of DVC astrocytes did not increase basal blood glucose nor protect against insulin\u2010induced hypoglycaemia. In male mice, chemogenetic DVC astrocyte activation did not alter glucose tolerance. In female mice, the initial glucose excursion was reduced in a glucose tolerance test, suggesting enhanced glucose absorption. Based on our data and published work, we propose that DVC astrocytes may play an indispensable homeostatic role, that is, are necessary to maintain the function of glucoregulatory neuronal circuitry, but alone their bulk activation is not sufficient to result in adaptive glucoregulatory responses. It is possible that there are state\u2010dependent effects and\/or DVC astrocyte subsets that have this specialised role, but this was unresolvable using the experimental approaches employed here.<\/jats:p>","DOI":"10.1111\/jne.13315","type":"journal-article","created":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T08:15:04Z","timestamp":1686298504000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Impact of chemogenetic activation of dorsal vagal complex astrocytes in mice on adaptive glucoregulatory responses"],"prefix":"10.1111","volume":"35","author":[{"given":"Alastair J.","family":"MacDonald","sequence":"first","affiliation":[{"name":"Institute of Biomedical and Clinical Sciences, University of Exeter Medical School  Exeter UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine R.","family":"Pye","sequence":"additional","affiliation":[{"name":"Institute of Biomedical and Clinical Sciences, University of Exeter Medical School  Exeter UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Beall","sequence":"additional","affiliation":[{"name":"Institute of Biomedical and Clinical Sciences, University of Exeter Medical School  Exeter UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5261-7465","authenticated-orcid":false,"given":"Kate L. 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