{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T02:06:07Z","timestamp":1778637967702,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T00:00:00Z","timestamp":1734480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["25191"],"award-info":[{"award-number":["25191"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Pituitary tumours (PT) are formed in the pituitary gland, a small gland situated at the base of the brain. These tumours can be categorized according to their histological origin and hormone production. In surgical series, non\u2010functioning PT are the commonest subtype, followed by functioning somatotroph and corticotroph tumours. Different somatic alterations have been implicated in the pathogenesis of these tumours and the objective of our study was to expand our previous new finding of\n                    <jats:italic>KRAS<\/jats:italic>\n                    pathogenic genetic variants in pituitary tumours. We conducted a digital polymerase chain reaction (PCR) analysis on formalin\u2010fixed paraffin\u2010embedded tissue blocks belonging to 189 patients. The results showed that, from the 184 pituitary tumours with good quality samples, 13 tumours (7.1%) presented mutant\n                    <jats:italic>KRAS<\/jats:italic>\n                    . The median age of the mutated group was 47\u2009years old (range 19\u201377) and most patients with mutant\n                    <jats:italic>KRAS<\/jats:italic>\n                    tumours were from the female gender (61.5%, 8\/13) and non\u2010functioning subtype. For the first\u2010time, mutant\n                    <jats:italic>KRAS<\/jats:italic>\n                    in corticotroph and somatotroph tumours were detected, and the variants showed low allele frequencies. In conclusion, we demonstrated that pituitary tumours might have mutant\n                    <jats:italic>KRAS<\/jats:italic>\n                    , and these data were not previously described probably due to lack of sensitivity of previous technologies. By identifying these variants, even at minimal levels, we open doors to a deeper understanding of the tumour microenvironment, clonal evolution and potential therapeutic targets.\n                  <\/jats:p>","DOI":"10.1111\/jne.13484","type":"journal-article","created":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T22:10:03Z","timestamp":1734559803000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Using digital\n                    <scp>PCR<\/scp>\n                    to investigate the prevalence of\n                    <scp>\n                      <i>KRAS<\/i>\n                    <\/scp>\n                    variants in pituitary tumours"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2751-0986","authenticated-orcid":false,"given":"Veronica","family":"Aran","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Biomedicina do C\u00e9rebro Instituto Estadual do C\u00e9rebro Paulo Niemeyer, Secretaria Estadual de Sa\u00fade  Rio de Janeiro Brazil"},{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Anatomia Patol\u00f3gica, Faculdade de Medicina Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa","family":"Lamback","sequence":"additional","affiliation":[{"name":"Neuroendocrinology Research Center, Endocrinology Section, Medical School and Hospital Universit\u00e1rio Clementino Fraga Filho Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"},{"name":"Neuropathology and Molecular Genetics Laboratory Instituto Estadual do C\u00e9rebro Paulo Niemeyer, Secretaria Estadual de Sa\u00fade  Rio de Janeiro Brazil"},{"name":"Neuroendocrine Unit Instituto Estadual do C\u00e9rebro Paulo Niemeyer, Secretaria Estadual de Sa\u00fade  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renan","family":"Lyra Miranda","sequence":"additional","affiliation":[{"name":"Neuropathology and Molecular Genetics Laboratory Instituto Estadual do C\u00e9rebro Paulo Niemeyer, Secretaria Estadual de Sa\u00fade  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandro","family":"Guterres","sequence":"additional","affiliation":[{"name":"Neuropathology and Molecular Genetics Laboratory Instituto Estadual do C\u00e9rebro Paulo Niemeyer, Secretaria Estadual de Sa\u00fade  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"Souza Barbosa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biomedicina do C\u00e9rebro Instituto Estadual do C\u00e9rebro Paulo Niemeyer, Secretaria Estadual de Sa\u00fade  Rio de Janeiro Brazil"},{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Anatomia Patol\u00f3gica, Faculdade de Medicina Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leila","family":"Chimelli","sequence":"additional","affiliation":[{"name":"Neuropathology and Molecular Genetics Laboratory Instituto Estadual do C\u00e9rebro Paulo Niemeyer, Secretaria Estadual de Sa\u00fade  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f4nica Roberto","family":"Gadelha","sequence":"additional","affiliation":[{"name":"Neuroendocrinology Research Center, Endocrinology Section, Medical School and Hospital Universit\u00e1rio Clementino Fraga Filho Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"},{"name":"Neuropathology and Molecular Genetics Laboratory Instituto Estadual do C\u00e9rebro Paulo Niemeyer, Secretaria Estadual de Sa\u00fade  Rio de Janeiro Brazil"},{"name":"Neuroendocrine Unit Instituto Estadual do C\u00e9rebro Paulo Niemeyer, Secretaria Estadual de Sa\u00fade  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivaldo","family":"Moura Neto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biomedicina do C\u00e9rebro Instituto Estadual do C\u00e9rebro Paulo Niemeyer, Secretaria Estadual de Sa\u00fade  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,18]]},"reference":[{"key":"e_1_2_11_2_1","volume-title":"Central Nervous System Tumours","author":"World Health Organization, WHO Classification of Tumours Editorial 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