{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T17:53:45Z","timestamp":1777658025222,"version":"3.51.4"},"reference-count":86,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1S10OD023587\u201001"],"award-info":[{"award-number":["1S10OD023587\u201001"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DP2MH119423"],"award-info":[{"award-number":["DP2MH119423"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21NS134228"],"award-info":[{"award-number":["R21NS134228"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1749500"],"award-info":[{"award-number":["1749500"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Among contributors to diffusible signaling are portal systems which join two capillary beds through connecting veins. Portal systems allow diffusible signals to be transported in high concentrations directly from one capillary bed to the other without dilution in the systemic circulation. Two portal systems have been identified in the brain. The first was discovered almost a century ago and connects the median eminence to the anterior pituitary gland. The second was discovered a few years ago and links the suprachiasmatic nucleus to the organum vasculosum of the lamina terminalis, a sensory circumventricular organ (CVO). Sensory CVOs bear neuronal receptors for sensing signals in the fluid milieu. They line the surface of brain ventricles and bear fenestrated capillaries thereby lacking blood\u2013brain barriers. It is not known whether the other sensory CVOs, namely the subfornical organ (SFO), and area postrema (AP) form portal neurovascular connections with nearby parenchymal tissue. To preserve the integrity of the vasculature of CVOs and their adjacent neuropil, we combined iDISCO clearing and light\u2010sheet microscopy to acquire volumetric images of blood vessels and traced the vasculature in two experiments. In the first, the whole brain vasculature was registered to the Allen Brain Atlas in order to identify the nuclei to which the SFO and AP are attached. In the second study, regionally specified immunolabeling was used to identify the attachment sites and vascular connections between the AP, and the SFO to their respective parenchymal attachment sites. There are venous portal pathways linking the capillary vessels of the SFO and the posterior septal nuclei, namely the septofimbrial nucleus and the triangular nucleus of the septum. Unlike the arrangement of portal vessels, the AP and the nucleus of the solitary tract share a common capillary bed. Taken together, the results reveal that all three sensory CVOs bear direct capillary connections to adjacent neuropil, providing a direct route for diffusible signals to travel from their source to their targets.<\/jats:p>","DOI":"10.1111\/jne.13490","type":"journal-article","created":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T05:21:44Z","timestamp":1736227304000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Capillary connections between sensory circumventricular organs and adjacent parenchyma enable local volume transmission"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0337-8802","authenticated-orcid":false,"given":"Yifan","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Psychology Columbia University  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2959-2885","authenticated-orcid":false,"given":"Yannan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Columbia University  New York New York USA"},{"name":"Department of Biomedical Engineering Columbia University  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raju","family":"Tomer","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Columbia University  New York New York USA"},{"name":"Department of Biomedical Engineering Columbia University  New York New York USA"},{"name":"Zuckerman Institute Columbia University  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rae","family":"Silver","sequence":"additional","affiliation":[{"name":"Department of Psychology Columbia University  New York New York USA"},{"name":"Zuckerman Institute Columbia University  New York New York USA"},{"name":"Department of Neuroscience Barnard College  New York New York USA"},{"name":"Department of Pathology and Cell Biology Columbia University Medical School  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1515\/revneuro\u20102015\u20100007"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3003748"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2451"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582\u2010020\u20100312\u2010z"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.0619\u201021.2021"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-374245-2.00015-2"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1987.120"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo11080494"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401\u2010010\u20100743\u20105"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201025793\u2010z"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adn8350"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-25519-3_4"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901330304"},{"key":"e_1_2_9_15_1","first-page":"333","volume-title":"International Review of Cytology","author":"Dellmann HD","year":"1979"},{"key":"e_1_2_9_16_1","first-page":"562","volume-title":"Circumventricular organs. The Human Nervous System","author":"McKinley MJ","year":"2003"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00711065"},{"key":"e_1_2_9_18_1","volume-title":"Dorland's Illustrated Medical Dictionary","author":"Dorland WAN","year":"2020"},{"issue":"1","key":"e_1_2_9_19_1","first-page":"88","article-title":"A portal circulation from the pituitary to the hypothalamic region","volume":"65","author":"Popa G","year":"1930","journal-title":"J Anat"},{"issue":"2","key":"e_1_2_9_20_1","first-page":"227","article-title":"Hypophysio\u2010portal vessels and their colloid accompaniment","volume":"67","author":"Popa GT","year":"1933","journal-title":"J Anat"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1969.49.2.240"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007\u20101139"},{"issue":"4371","key":"e_1_2_9_23_1","first-page":"559","article-title":"Neural control of the pituitary gland","volume":"2","author":"Harris GW","year":"1955","journal-title":"Br Med J"},{"key":"e_1_2_9_24_1","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1002\/j.2040-4603.2016.tb00708.x","article-title":"Hypothalamus\u2010pituitary\u2010thyroid axis","volume":"6","author":"Ortiga\u2010Carvalho TM","year":"2011","journal-title":"Compr Physiol"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00003.2011"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102826.2002.00798.x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/07487304231225706"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1530\/joe\u201015\u20100113"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010physiol\u2010021909\u2010135821"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.13446"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology12040508"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature19756"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/jne.12689"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00212226"},{"key":"e_1_2_9_35_1","volume-title":"Allen Mouse Brain Atlas","author":"Allen Institute for Brain Science","year":"2004"},{"key":"e_1_2_9_36_1","volume-title":"Paxinos and Franklin's the Mouse Brain in Stereotaxic Coordinates","author":"Paxinos G","year":"2019"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u2010079X.1989.tb00667.x"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.05.007"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306\u20104522(92)90525\u20107"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201030020\u20109"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.123"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010019\u20100501\u20100"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.113491"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fninf.2014.00013"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593\u2010021\u201000904\u20107"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010020\u20102649\u20102"},{"key":"e_1_2_9_48_1","first-page":"51","article-title":"Data structures for statistical computing in python","volume":"445","author":"McKinney W","year":"2010","journal-title":"SciPy"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.21105\/joss.01026"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1109\/MCSE.2007.55"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.21105\/joss.03021"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901860408"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-1302-4_1"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901160102"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2007.06.002"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006\u20108993(86)91161\u20103"},{"issue":"6","key":"e_1_2_9_57_1","first-page":"427","article-title":"The human subfornical organ: an anatomic and ultrastructural study","volume":"14","author":"Mark MH","year":"1984","journal-title":"Ann Clin Lab Sci"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00331260"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.2739\/kurumemedj.36.91"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201030533\u20103"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2021.02.006"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.01.028"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201014786\u2010z"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010020\u20100792\u20101"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1159\/000142455"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014\u20104886(68)90150\u20107"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00319249"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902340306"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1139\/y91\u2010152%M1954559"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1159\/000158652"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2021.691711"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1369\/0022155415575027"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.25470"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441\u2010015\u20102207\u20107"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2007.09.023"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1111\/jne.13245"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1159\/000143768"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/90.3.497"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.02\u201008\u201001043.1982"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901730107"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031\u20109384(92)90431\u2010Z"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306\u20104522(98)00026\u20108"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42255\u2010022\u201000606\u20109"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.1177\/1073858407311100"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902340306"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2019.10.010"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adh8488"}],"updated-by":[{"DOI":"10.1111\/jne.70192","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000}}],"container-title":["Journal of Neuroendocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jne.13490","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T23:46:25Z","timestamp":1744069585000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jne.13490"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,7]]},"references-count":86,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1111\/jne.13490"],"URL":"https:\/\/doi.org\/10.1111\/jne.13490","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/JNE.13490\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNE.13490\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNE.13490\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNE.13490\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNE.13490\/v2\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNE.13490\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNE.13490\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNE.13490\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNE.13490\/v2\/decision1","asserted-by":"object"}],"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.07.30.605849","asserted-by":"object"}]},"ISSN":["0953-8194","1365-2826"],"issn-type":[{"value":"0953-8194","type":"print"},{"value":"1365-2826","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,7]]},"assertion":[{"value":"2024-08-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13490"}}