{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T04:56:43Z","timestamp":1789102603201,"version":"build-2803163510"},"reference-count":84,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,3,8]],"date-time":"2025-03-08T00:00:00Z","timestamp":1741392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The hypothalamic suprachiasmatic nucleus (SCN) hosts the central circadian pacemaker and regulates daily rhythms in physiology and behavior. The SCN is composed of peptidergic neuron populations expressing arginine vasopressin (AVP) and vasoactive intestinal polypeptide (VIP), as well as glial cells. Patients with Prader\u2013Willi Syndrome (PWS) commonly experience circadian disturbances, which are particularly evident in their sleep\/wake patterns. Using publicly available single\u2010cell RNA sequencing data, we assessed the cell\u2010type specificity of PWS\u2010causative genes in murine SCN, which revealed the differential presence of PWS\u2010related genes in glial and neural subpopulations. We then investigated neurons and glial cells in the SCN using immunohistochemistry in the postmortem hypothalami of PWS subjects and matched controls. We profiled neural populations characterized by AVP and VIP, astroglia characterized by glial fibrillary acid protein (GFAP), and microglia marked by ionized calcium\u2010binding adapter molecule 1 (Iba1) and NADPH oxidase 2 (NOX2). Our analysis revealed an increased total number, neuronal density, and relative staining intensity of AVP\u2010containing neurons in the PWS compared to controls while VIP\u2010containing cells were unaltered. In contrast, GFAP\u2010expressing astroglial cells were significantly lower in PWS subjects. Moreover, we did not detect any differences in microglia between PWS subjects and controls. Collectively, our findings show that PWS selectively affects AVP\u2010containing neurons and GFAP\u2010expressing astrocytes in the SCN. As each of these cell populations can affect the daily rhythmicity of the SCN biological clock machinery, the disruption of these cells may contribute to the circadian disturbances in patients with PWS.<\/jats:p>","DOI":"10.1111\/jne.70015","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:21:10Z","timestamp":1741738870000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Selective changes in vasopressin neurons and astrocytes in the suprachiasmatic nucleus of Prader\u2013Willi syndrome subjects"],"prefix":"10.1111","volume":"37","author":[{"given":"Felipe","family":"Correa\u2010da\u2010Silva","sequence":"first","affiliation":[{"name":"Department of Endocrinology and Metabolism, Amsterdam University Medical Center, Location AMC University of Amsterdam  Amsterdam The Netherlands"},{"name":"Amsterdam Gastroenterology Endocrinology and Metabolism  Amsterdam The Netherlands"},{"name":"Department of Clinical Chemistry, Laboratory of Endocrinology Amsterdam University Medical Center, Location AMC  Amsterdam The Netherlands"},{"name":"Netherlands Institute for Neuroscience  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jari B.","family":"Berkhout","sequence":"additional","affiliation":[{"name":"Dept. of Medicine Div. 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