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The properties of isolated GABAergic postsynaptic currents in GnRH neurons were used to generate representative model conductances of 1, 2, 5, and 10\u2009nS, with decay time constants representing prepubertal and adult mice (7 vs. 10\u2009ms). These conductances were applied to GnRH neurons from each experimental group using dynamic clamp, and response was measured. Neither development nor PNA altered the response of GnRH neurons to small conductances (1 or 2 nS), and these conductances did not initiate action potentials. In response to the 5 nS conductance, dynamic\u2010clamp\u2010induced postsynaptic potentials were larger in 3\u2010week\u2010old controls versus 3\u2010week\u2010old PNA mice at the 7\u2009ms decay time constant and larger than vehicle\u2010treated (VEH) adults at the 10\u2009ms decay time constant. In response to larger conductances, only seven of 78 GnRH neurons from adults generated action potentials, whereas 14 of 73 GnRH neurons from 3\u2010week\u2010old females did. Interestingly, an altered action potential waveform was observed only in 3\u2010week\u2010old PNA females. The changes in GnRH neuron intrinsic properties occurring with development and PNA treatment result in differential responses to the same physiologic GABA input and may contribute to the action potential firing changes previously reported in this model.<\/jats:p>","DOI":"10.1111\/jne.70144","type":"journal-article","created":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T06:50:12Z","timestamp":1770447012000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Response of gonadotropin\u2010releasing hormone neurons from female mice to dynamic\u2010clamp\u2010simulated\n                    <scp>GABAergic<\/scp>\n                    conductances across development and after prenatal androgenization"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0870-2149","authenticated-orcid":false,"given":"Jennifer","family":"Jaime","sequence":"first","affiliation":[{"name":"Department of Molecular and Integrative Physiology University of Michigan  Ann Arbor Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. Anthony","family":"DeFazio","sequence":"additional","affiliation":[{"name":"Department of Molecular and Integrative Physiology University of Michigan  Ann Arbor Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne M.","family":"Moenter","sequence":"additional","affiliation":[{"name":"Department of Molecular and Integrative Physiology University of Michigan  Ann Arbor Michigan USA"},{"name":"Department of Internal Medicine University of Michigan  Ann Arbor Michigan USA"},{"name":"Department of Obstetrics &amp; Gynecology University of Michigan  Ann Arbor Michigan USA"},{"name":"Department of the Reproductive Sciences Program University of Michigan  Ann Arbor Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/8.suppl_2.51"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-128-1-509"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-109-2-376"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dml017"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1385\/ENDO:30:1:3"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2011.12.007"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2019.110561"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2003-43304"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jne.13093"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-024-00511-3"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-30-4-435"},{"issue":"1","key":"e_1_2_10_13_1","first-page":"119","article-title":"Accelerated 24\u2010hour luteinizing hormone pulsatile activity in adolescent girls with ovarian hyperandrogenism: relevance to the developmental phase of polycystic ovarian syndrome","volume":"79","author":"Apter D","year":"1994","journal-title":"J Clin Endocrinol Metab"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecl.2020.10.003"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1740001"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.105.039800"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2010.05.017"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010-0823"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308058101"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1415038112"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2017-00768"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2304-17.2018"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2002-0163"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2011.02145.x"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1523\/ENEURO.0362-22.2022"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/jne.13373"},{"key":"e_1_2_10_27_1","volume-title":"Voltage\u2010Gated Calcium Channel Activity of Gonadotropin\u2010Releasing Hormone (GnRH) Neurons Is Altered by Prenatal Androgen Exposure in Female Mice","author":"Chen X","year":"2025"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.141.1.7279"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(94)90031-0"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1670-09.2009"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470721896"},{"key":"e_1_2_10_32_1","unstructured":"GohelD.flextable: functions for tabular reporting (R package version 0.5.11).2020. 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