{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:54:18Z","timestamp":1774227258409,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF20SA0061466"],"award-info":[{"award-number":["NNF20SA0061466"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100031879","name":"Steno Diabetes Center Aarhus","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100031879","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Neuroendocrine tumors (NETs) are rare tumors that may arise in the small intestine (siNET) or pancreas (pNET). The tumors have heterogeneous clinical characteristics, and diagnosis may be challenging. Current non\u2010invasive biomarkers perform suboptimally, and novel biomarkers are highly needed. We aimed to investigate the use of RNA aptamer panels, selected by the APTASHAPE technology, in distinguishing healthy individuals from individuals with NET as well as discriminating siNET from pNET. Plasma samples from 68 individuals with NET and 24 healthy individuals were collected and randomly divided into a development set and a test set. A panel of aptamers recognizing disease\u2010specific plasma proteins was selected in the development set, and the accuracy for disease prediction was evaluated in the test set. The aptamer panels demonstrated high discriminative power, accurately distinguishing between healthy individuals and NET patients with AUCs of 0.74 and 0.87 in the development set and test set, respectively. Furthermore, the aptamer panels were able to differentiate between siNET and pNET patients with AUCs of 0.72 in both the development set and the test set. In addition, mass spectrometry analysis identified ITIH2 and IGHG3 as potential novel biomarkers for the diagnosis of NET. The APTASHAPE platform is a capable tool for selecting protein biomarker\u2010directed aptamer panels for the diagnosis of NET and discrimination between siNET and pNET. Furthermore, the identification of ITIH2 and IGHG3 highlights the potential of APTASHAPE to identify novel protein biomarkers in NET.<\/jats:p>","DOI":"10.1111\/jne.70146","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T03:19:52Z","timestamp":1773026392000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biomarker\u2010directed aptamer panels for diagnosis and differentiation of neuroendocrine tumors"],"prefix":"10.1111","volume":"38","author":[{"given":"Mikkel Breinholt","family":"Kj\u00e6r","sequence":"first","affiliation":[{"name":"Department of Hepatology and Gastroenterology Aarhus University Hospital  Aarhus Denmark"},{"name":"Department of Clinical Medicine Aarhus University  Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asger Givskov","family":"J\u00f8rgensen","sequence":"additional","affiliation":[{"name":"J\u00f8rgen Kjems Lab, Interdisciplinary Nanoscience Centre (iNANO), Department of Molecular Biology and Genetics Aarhus University  Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00f8ren","family":"Fjelstrup","sequence":"additional","affiliation":[{"name":"J\u00f8rgen Kjems Lab, Interdisciplinary Nanoscience Centre (iNANO), Department of Molecular Biology and Genetics Aarhus University  Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel Miotto","family":"Dupont","sequence":"additional","affiliation":[{"name":"J\u00f8rgen Kjems Lab, Interdisciplinary Nanoscience Centre (iNANO), Department of Molecular Biology and Genetics Aarhus University  Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claus","family":"Bus","sequence":"additional","affiliation":[{"name":"J\u00f8rgen Kjems Lab, Interdisciplinary Nanoscience Centre (iNANO), Department of Molecular Biology and Genetics Aarhus University  Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stine Karlsen","family":"Oversoe","sequence":"additional","affiliation":[{"name":"Department of Hepatology and Gastroenterology Aarhus University Hospital  Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens","family":"Kelsen","sequence":"additional","affiliation":[{"name":"Department of Hepatology and Gastroenterology Aarhus University Hospital  Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f8rgen","family":"Kjems","sequence":"additional","affiliation":[{"name":"J\u00f8rgen Kjems Lab, Interdisciplinary Nanoscience Centre (iNANO), Department of Molecular Biology and Genetics Aarhus University  Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henning","family":"Gr\u00f8nb\u00e6k","sequence":"additional","affiliation":[{"name":"Department of Hepatology and Gastroenterology Aarhus University Hospital  Aarhus Denmark"},{"name":"Department of Clinical Medicine Aarhus University  Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2017.0589"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-45215-8_3"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1530\/EC-16-0043"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics10110881"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1530\/ERC-17-0269"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hpb.2018.09.016"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000503833"},{"issue":"2","key":"e_1_2_7_9_1","article-title":"The role of serum 5\u2010HIAA as a predictor of progression and an alternative to 24\u2010h urine 5\u2010HIAA in well\u2010differentiated 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