{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T14:42:16Z","timestamp":1788273736883,"version":"build-2803163510"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:00:00Z","timestamp":1773100800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002426","name":"Fondazione Telethon","doi-asserted-by":"publisher","award":["GGP19103"],"award-info":[{"award-number":["GGP19103"]}],"id":[{"id":"10.13039\/501100002426","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001673","name":"Fondation J\u00e9r\u00f4me Lejeune","doi-asserted-by":"publisher","award":["GRT#2109"],"award-info":[{"award-number":["GRT#2109"]}],"id":[{"id":"10.13039\/501100001673","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Oxytocin plays an emerging role in vascular regulation and neuroprotection, but its effect on brain endothelial cells and blood\u2013brain barrier functionality is not fully defined. To assess oxytocin and vasopressin receptor expression in brain endothelial cells and to evaluate the impact of oxytocin on endothelial barrier integrity under physiological (normoxic) and low\u2010oxygen (hypoxic) conditions we used bEnd.3 brain endothelial cells. Receptor expression was evaluated by real\u2010time PCR and RNAscope. Oxytocin treatment was applied under normoxic and hypoxic conditions and Transendothelial Electrical Resistance and tight junction proteins expression (claudin\u20105, zonula occludens\u20101) were analyzed. Our results indicate that 1) bEnd.3 cells express oxytocin and V1a receptors. 2) Activation of the oxytocin receptor enhanced brain endothelial barrier integrity by increasing claudin\u20105 expression and its localization at the cell surface, without affecting zonula occludens\u20101.3) Under hypoxic conditions, oxytocin preserved Transendothelial Electrical Resistance and claudin\u20105 expression at the cell membrane, thereby preventing endothelial barrier impairment. Our findings demonstrate that oxytocin receptor signaling enhances and preserves brain endothelial barrier function, underscoring the relevance of oxytocin in neurovascular regulation and its therapeutic potential in blood\u2013brain barrier dysfunction.<\/jats:p>","DOI":"10.1111\/jne.70152","type":"journal-article","created":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T23:19:51Z","timestamp":1773184791000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Oxytocin supports the barrier integrity of brain endothelial cells via oxytocin receptors"],"prefix":"10.1111","volume":"38","author":[{"given":"Camilla","family":"Paolini","sequence":"first","affiliation":[{"name":"Institute of Neuroscience, National Research Council (CNR)  Vedano al Lambro Monza and Brianza Italy"},{"name":"University of Milano\u2010Bicocca  Milan Italy"},{"name":"NeuroMI Milan Center for Neuroscience  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2771-4758","authenticated-orcid":false,"given":"Francesca","family":"Piacentini","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience, National Research Council (CNR)  Vedano al Lambro Monza and Brianza Italy"},{"name":"University of Milano\u2010Bicocca  Milan Italy"},{"name":"NeuroMI Milan Center for Neuroscience  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Amato","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience, National Research Council (CNR)  Vedano al Lambro Monza and Brianza Italy"},{"name":"NeuroMI Milan Center for Neuroscience  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3387-2190","authenticated-orcid":false,"given":"Marta","family":"Busnelli","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience, National Research Council (CNR)  Vedano al Lambro Monza and Brianza Italy"},{"name":"NeuroMI Milan Center for Neuroscience  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bice","family":"Chini","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience, National Research Council (CNR)  Vedano al Lambro Monza and Brianza Italy"},{"name":"NeuroMI Milan Center for Neuroscience  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.15372"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100617-062608"},{"key":"e_1_2_10_4_1","first-page":"39","article-title":"Structural and functional aspects of the blood\u2010brain barrier","volume":"61","author":"Begley DJ","year":"2003","journal-title":"Progr Drug Res Fortschritte der Arzneimittelforschung"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12987-022-00312-4"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.57.2.4"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2018.06.033"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-018-1815-1"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000511552"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232315271"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2020.594672"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells13080660"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24544"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808698106"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0263369"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202200010RR"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2020.1851897"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/healthcare12080835"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2023.176003"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/endocr\/bqac111"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2022.102328"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.140.3.6546"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-0528.2003.02259.x"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-06-0024"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0707609"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2009.06.022"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.04.021"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2017.10.022"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2011.09.004"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2020.574499"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awaf112"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awab055"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.10.083"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b12-00915"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.113.202994"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2024.06.010"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0268860"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0187017"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.060693"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1290\/1543-706X(2003)039<0313:COMBME>2.0.CO;2"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12987-021-00297-6"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.120.000146"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.135.4.7925112"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0262152"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/1440-1681.12587"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2014.00352"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2021.1121"},{"key":"e_1_2_10_48_1","first-page":"337","article-title":"Pre\u2010ischemic oxytocin treatment alleviated neuronal injury via suppressing NF\u2010kappaB, MMP\u20109, and apoptosis regulator proteins in a mice model of stroke","volume":"24","author":"Momenabadi S","year":"2022","journal-title":"Cell J"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/micc.12229"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2009-0624"}],"container-title":["Journal of Neuroendocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jne.70152","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jne.70152","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jne.70152","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:32:27Z","timestamp":1774225947000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jne.70152"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":49,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/jne.70152"],"URL":"https:\/\/doi.org\/10.1111\/jne.70152","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/JNE.70152\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNE.70152\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNE.70152\/v1\/review1","asserted-by":"object"}]},"ISSN":["0953-8194","1365-2826"],"issn-type":[{"value":"0953-8194","type":"print"},{"value":"1365-2826","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"2025-07-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70152"}}