{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:14:29Z","timestamp":1788480869482,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Si1487\/3\u20101"],"award-info":[{"award-number":["Si1487\/3\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["TRR\/SF209 (B04)"],"award-info":[{"award-number":["TRR\/SF209 (B04)"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Nucleoporins (NUPs) constitute the nuclear pore complex (NPC) and are essentially involved in nuclear transport, chromatin organization, and context\u2010dependent gene regulation. However, the role of NUPs in neuroendocrine (tumor)biology and related diagnostic potential is poorly defined. In this study, we comparatively analyzed immunohistochemical expression patterns of NUP98 and NUP153 (sharing structural and functional similarities) across a large variety of human tissues and tumors (total\n                    <jats:italic>N<\/jats:italic>\n                    \u2009&gt;\u2009600), with a focus on neuroendocrine neoplasms (NENs (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009361)). While both NUPs showed a nuclear rim accentuated staining pattern, NUP98 exhibited ubiquitous and NUP153 a striking cell\u2010 and tissue\/tumor\u2010type\u2010dependent immunoreactivity. More specifically, NUP153 immunoreactivity was consistently detectable in neuroendocrine tissues (e.g., pancreatic islets) and retained in neuroendocrine tumors (NETs) of the pancreas, lung, appendix, and small intestine, but almost completely absent in neuroendocrine carcinomas (NECs) and non\u2010neuroendocrine carcinomas. Loss of NUP153 staining correlated with poorer clinical outcomes in pancreatic NETs. Further analyses revealed that NUP153 messenger ribonucleic acid (mRNA) and total protein levels were not significantly different between NETs and non\u2010neuroendocrine carcinomas, as evaluated by quantitative real\u2010time polymerase chain reaction and targeted proteomics. Differential isoform usage was ruled out by polymerase chain reaction and sequencing as another possible explanation for the discriminative immunohistochemical findings. Finally, a high density of post\u2010translational modifications (PTMs) within the antigen sequence could be discovered and indicated PTM\u2010dependent detection as the likely cause of differential staining. Collectively, our findings suggest that NUP153 is a differentiation\u2010dependent and PTM\u2010sensitive immunohistochemical marker in NENs with diagnostic and prognostic potential. It also emphasizes the importance of orthogonal molecular analyses for correct interpretation of IHC stainings.\n                  <\/jats:p>","DOI":"10.1111\/jne.70240","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T02:35:41Z","timestamp":1786588541000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["NUP153 immunoreactivity in neuroendocrine neoplasms supports the diagnosis of\n                    <scp>NET<\/scp>\n                    versus\n                    <scp>NEC<\/scp>\n                    and is modulated by post\u2010translational modifications"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7362-0209","authenticated-orcid":false,"given":"Sven","family":"Mattern","sequence":"first","affiliation":[{"name":"Institute of Pathology University Hospital T\u00fcbingen  T\u00fcbingen 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Thoraxklinik at University Hospital Heidelberg  Heidelberg Germany"},{"name":"Translational Lung Research Center (TLRC) German Center for Lung Research (DZL)  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Lehmann\u2010Koch","sequence":"additional","affiliation":[{"name":"Institute for Pathology SLK\u2010Kliniken Heilbronn  Heilbronn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Goeppert","sequence":"additional","affiliation":[{"name":"Institute for Pathology and Neuropathology RKH Hospital Ludwigsburg  Ludwigsburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arne","family":"Warth","sequence":"additional","affiliation":[{"name":"\u00dcGP MVZ Institute for Pathology, Dermatopathology, Cytology, and Molecular Pathology GbR  Wetzlar 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