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Prenatally androgenized (PNA) mice mimic neuroendocrine PMOS symptoms including high LH\u2010pulse frequency. GnRH neurons from adult PNA mice have a higher spontaneous firing rate than those from vehicle (VEH) mice; this is reversed in peripubertal mice despite more excitatory inputs at both ages. We hypothesized voltage\u2010gated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    currents (\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>Ca<\/jats:sub>\n                    ) help set intrinsic excitability of GnRH neurons and are altered by development and\/or PNA treatment. Whole\u2010cell patch\u2010clamp measurements were made of GnRH neuron\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>Ca<\/jats:sub>\n                    and excitability in 3 week\u2010old and adult VEH and PNA mice. PNA treatment increased\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>Ca<\/jats:sub>\n                    density and depolarized the\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>Ca<\/jats:sub>\n                    \u2010half\u2010inactivation potential at both ages. In adults, the half\u2010activation potential of the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    conductance was depolarized, and the half\u2010inactivation time of the fast\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>Ca<\/jats:sub>\n                    component was increased regardless of PNA treatment. There was an age\u2010related change in firing response to current injections driven primarily by more firing in 3\u2009week\u2010old VEH mice; 3\u2009week\u2010old PNA mice had adult\u2010like responses. Blocking small\u2010conductance Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010activated K\n                    <jats:sup>+<\/jats:sup>\n                    current with apamin increased GnRH neuron firing rate except in adult PNA mice. Apamin changed the post\u2010spike\u2010train membrane response from hyperpolarization to depolarization; during development, this net effect of apamin was smaller in PNA mice. In summary, GnRH neurons from PNA mice have increased\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>Ca<\/jats:sub>\n                    and altered\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>Ca<\/jats:sub>\n                    kinetics. Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010activated K\n                    <jats:sup>+<\/jats:sup>\n                    currents are less prominent in GnRH neurons from adult PNA mice, perhaps contributing to their increased spontaneous firing activity.\n                  <\/jats:p>","DOI":"10.1111\/jne.70268","type":"journal-article","created":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T00:20:24Z","timestamp":1789777224000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Voltage\u2010gated calcium channel activity of gonadotropin\u2010releasing hormone (\n                    <scp>GnRH<\/scp>\n                    ) neurons is altered by age and by prenatal androgen exposure in female mice"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9445-3240","authenticated-orcid":false,"given":"Xi","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Molecular &amp; Integrative Physiology University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0870-2149","authenticated-orcid":false,"given":"Jennifer","family":"Jaime","sequence":"additional","affiliation":[{"name":"The Neuroscience Graduate Program University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7302-7528","authenticated-orcid":false,"given":"R. Anthony","family":"DeFazio","sequence":"additional","affiliation":[{"name":"Department of Molecular &amp; Integrative Physiology University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9812-0497","authenticated-orcid":false,"given":"Suzanne M.","family":"Moenter","sequence":"additional","affiliation":[{"name":"Department of Molecular &amp; Integrative Physiology University of Michigan  Ann Arbor Michigan USA"},{"name":"Department of Internal Medicine University of Michigan  Ann Arbor Michigan USA"},{"name":"Obstetrics and Gynecology University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.100883"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo\u2010111\u20105\u20101737"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.130.1.1727719"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo\u2010109\u20102\u2010376"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo\u2010125\u20102\u2010917"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jne.13093"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo\u2010129\u20103\u20101175"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(26)00717\u20108"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.85.11.6992"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.83.2.4604"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2011.12.007"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.79.1.8027216"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2004\u20102359"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2008\u20102606"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958\u2010017\u20100282\u20101"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010019\u20100666\u20101"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.4067\/S0034\u201098872012000700015"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/17.10.2573"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0015\u20100282(97)81873\u20100"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1023\/a:1014075016956"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.k09e\u2010205"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308058101"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2012\u20101954"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1415038112"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2017\u201000768"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2002\u20100163"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2304\u201017.2018"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/jne.13373"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1523\/ENEURO.0362\u201022.2022"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2355\u201022.2023"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.6156\u201009.2010"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1523\/ENEURO.0171\u201018.2018"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.70.5.549"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.110.066100"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.70046"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00822"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2025.130862"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424\u2010023\u201002891\u2010z"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.08.039"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a003947"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0166\u20102236(00)01738\u20100"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.6256\u201009.2010"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.019265"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003\u20100213"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2962\u201009.2009"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/wmts.102"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0340903"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem\u201065\u20102\u2010233"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2012.12.021"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010\u20100385"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1210\/endocr\/bqz038"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3171\u201006.2006"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1210\/endocr\/bqad194"},{"key":"e_1_2_9_55_1","d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