{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T08:11:41Z","timestamp":1749197501490,"version":"3.40.5"},"reference-count":60,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T00:00:00Z","timestamp":1691020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Peripheral Nervous Sys"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aims<\/jats:title><jats:p>Cisplatin is a chemotherapeutic agent for many types of cancer. The neurotoxicity of cisplatin includes neuropathy and allodynia. We aimed to study structural changes by using CYM54\u201078, attenuating cisplatin\u2010induced neuropathy and blocking the pathogenesis in neurons, and promoting axonal regeneration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>TEM (transmission electron microscopy) was used to distinguish ultrastructural changes in dorsal root ganglion (DRG) and dorsal rootlets (DR) between rats treated with cisplatin alone and rats co\u2010treated with cisplatin and sphingosine \u20101\u2010phosphate receptor2 (S1P2) agonist, CYM\u20105478.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In DRG of rats treated with cisplatin alone, TEM micrographs showed necrosis and apoptotic cells. Neuronal cytoplasm showed numerous vacuole (stage C) and swelling (stage B\u2794C) mitochondrial degeneration. Neurons in DRG from cisplatin+CYM\u20105478 group showed a higher percentage of healthy mitochondria (from 5.3% to 75.6%) than those treated with cisplatin alone. DR of cisplatin only group showed abnormal axoplasm, axolemma, and focal detached myelin sheaths, especially in A\u03b4 (fast pain) and A\u03b2 (touch) fibers, and revealed collateral branches that sprouted from A\u03b2 fibers, which is characteristic of allodynia. Moreover, vasoconstriction was observed in DRG and DR. Rats in cisplatin+CYM\u20105478 group showed not only fewer abnormal structures than those in cisplatin only group, but also showed Bands of B\u00fcngner and onion bulb\u2010like structures, which are characteristic of nerve regeneration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Interpretation<\/jats:title><jats:p>Together with our previous study, showed that CYM\u20105478 attenuated neuropathy and allodynia in a rat model of cisplatin\u2010induced neuropathy, these results suggest S1P2 agonists as a potential approach the for treatment of cancer due to the reduction of side effects of cisplatin.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jns.12582","type":"journal-article","created":{"date-parts":[[2023,7,23]],"date-time":"2023-07-23T02:24:50Z","timestamp":1690079090000},"page":"476-489","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Differences in the ultrastructure of neurons in the spinal ganglion and dorsal rootlet between rats treated with cisplatin only versus co\u2010administration with a sphingosine 1\u2010phosphate receptor 2 agonist in attenuating neuropathy and allodynia"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6115-9141","authenticated-orcid":false,"given":"Kanokporn","family":"Chayaburakul","sequence":"first","affiliation":[{"name":"Anatomy Unit, Faculty of Science Rangsit University  Meung, Pathum Tani Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei Yi","family":"Ong","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Yong Loo Lin School of Medicine National University Health System, National University of Singapore  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deron R.","family":"Herr","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Yong Loo Lin School of Medicine National University Health System, National University of Singapore  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phetnarin","family":"Kobutree","sequence":"additional","affiliation":[{"name":"Anatomy Unit, Faculty of Science Rangsit University  Meung, Pathum Tani Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kraisri","family":"Chantra","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Faculty of Medicine, King Chulalongkorn Memorial Hospital Chulalongkorn University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,8,3]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"196","volume-title":"Schwann Cells, Nervous Tissue: Color Textbook of Histology","author":"Gartner LP","year":"2007"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750\u20103639.1999.tb00229.x"},{"key":"e_1_2_9_4_1","first-page":"329","volume-title":"Nervous Tissue, a Text and 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