{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T22:33:48Z","timestamp":1785191628735,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T00:00:00Z","timestamp":1742256000000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Peripheral Nervous Sys"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background and Aims<\/jats:title><jats:p>At the neuromuscular junction (NMJ), the synapse between motor neurons and muscle fibers, reside perisynaptic Schwann cells (PSCs) which are specialized glia that regulate the maintenance and repair of this synapse. While we know how PSC morphology and numbers change in aging and various neuromuscular disorders that adversely affect the NMJ, the molecular mechanisms that alter PSC functions remain unknown. In this study, we investigated whether MEGF10 in PSCs modulates NMJ stability in developing, healthy young adult, middle\u2010aged, and axotomized mice. MEGF10 is a glial phagocytic receptor that is enriched in PSCs compared to other Schwann cells (SCs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We isolated PSCs from a transgenic reporter mouse line to assess <jats:italic>Megf10<\/jats:italic> expression at different ages and following nerve injury using qPCR. We then used a conditional mouse lacking <jats:italic>Megf10<\/jats:italic> in all SCs, including PSCs (<jats:italic>Megf10<\/jats:italic> SC\u2010KO mice). We examined NMJs and axonal debris clearance in <jats:italic>Megf10<\/jats:italic> SC\u2010KO mice using confocal microscopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that <jats:italic>Megf10<\/jats:italic> expression in PSCs peaks during development and decreases during aging and following denervation of NMJs. NMJs were morphologically normal in developing and young adult <jats:italic>Megf10<\/jats:italic> SC\u2010KO mice. This was not the case in middle\u2010aged <jats:italic>Megf10<\/jats:italic> SC\u2010KO mice, in which NMJs presented with fewer PSCs, decreased PSC coverage of the endplate, and decreased innervation in comparison to control mice. Following nerve injury\u2010induced damage, axonal debris at the NMJ was cleared faster in <jats:italic>Megf10<\/jats:italic> SC\u2010KO mice; yet, the rate of reinnervation was unchanged compared to control mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Interpretation<\/jats:title><jats:p>The data in this study suggest that MEGF10 in PSCs functions to maintain PSC number and NMJ innervation during aging. This study also suggests important roles for MEGF10 in mediating the clearance of axonal debris at NMJs following nerve injury.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jns.70014","type":"journal-article","created":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T11:16:15Z","timestamp":1742296575000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Loss of <scp>MEGF10<\/scp> Decreases the Number of Perisynaptic Schwann Cells and Innervation of Neuromuscular Junctions in Aging Mice"],"prefix":"10.1111","volume":"30","author":[{"given":"Devin","family":"Juros","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Cell Biology and Biochemistry Brown University  Providence Rhode Island USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert Louis","family":"Hastings","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Cell Biology and Biochemistry Brown University  Providence Rhode Island USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariane","family":"Pendragon","sequence":"additional","affiliation":[{"name":"Department of Neurobiology Duke University School of Medicine  Durham North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Kay","sequence":"additional","affiliation":[{"name":"Department of Neurobiology Duke University School of Medicine  Durham North Carolina USA"},{"name":"Department of Ophthalmology Duke University School of Medicine  Durham North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0375-4532","authenticated-orcid":false,"given":"Gregorio","family":"Valdez","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Cell Biology and Biochemistry Brown University  Providence Rhode Island USA"},{"name":"Center for Translational Neuroscience, Robert J. and Nancy D. Carney Institute for Brain Science, and Center on the Biology of Aging Brown University  Providence Rhode Island USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12776"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.14223"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.3850\u201015.2016"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2446"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2007.03.041"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2007.06.015"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10877"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200709083"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.995"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.27684"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13395\u2010024\u201000342\u20106"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddx189"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/2211\u20105463.13031"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.56935"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13981"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.3934\u201004.2004"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.004895"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2024.114615"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20335"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.34241"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03837"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2467"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3791\/54186"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3791\/58683"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2024.03.0072"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1970.sp009076"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.1748\u201018.2020"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896\u20106273(95)90246\u20105"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.3649\u201004.2004"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1710566114"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034640"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1736\u201020.2020"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/jns.12373"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a020487"}],"container-title":["Journal of the Peripheral Nervous System"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jns.70014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T00:58:51Z","timestamp":1743209931000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jns.70014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1111\/jns.70014"],"URL":"https:\/\/doi.org\/10.1111\/jns.70014","archive":["Portico"],"relation":{},"ISSN":["1085-9489","1529-8027"],"issn-type":[{"value":"1085-9489","type":"print"},{"value":"1529-8027","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"2024-06-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70014"}}