{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:37:52Z","timestamp":1761709072347,"version":"3.37.3"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,4,7]],"date-time":"2015-04-07T00:00:00Z","timestamp":1428364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004564","name":"Ministry of Education, Science and Technological Development of the Republic of Serbia","doi-asserted-by":"crossref","award":["173009"],"award-info":[{"award-number":["173009"]}],"id":[{"id":"10.13039\/501100004564","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Thyroid C\u2010cells produce calcitonin (CT), a hypocalcemic hormone, that acts as an inhibitor of bone resorption. In this study, we investigated the effects of tamoxifen (TAM) as a selective estrogen receptor modulator on thyroid C\u2010cells, trabecular bone and biochemical markers of bone metabolism in an animal model of androgen deficiency, represented by middle\u2010aged orchidectomized (Orx) rats. Fifteen\u2010month\u2010old male Wistar rats were divided into: Orx and sham\u2010operated (SO) groups. Rats from one Orx group were injected subcutaneously with TAM citrate (Orx + TAM; 0.3 mg\u00a0kg<jats:sup>\u22121<\/jats:sup> b.w.), while the rats from SO and a second Orx group received vehicle alone, once a day for 3 weeks. The peroxidase\u2013antiperoxidase method was applied for localization of CT in C\u2010cells. Thyroid C\u2010cells were morphometrically and ultrastructurally analyzed. An ImageJ image\u2010processing program was used to measure bone histomorphometric parameters. Blood serum samples were analyzed for CT, osteocalcin (OC), calcium (Ca<jats:sup>2+<\/jats:sup>) and phosphorus (P). Urinary Ca<jats:sup>2+<\/jats:sup> concentrations were measured. TAM treatment significantly increased thyroid C\u2010cell volume (<jats:italic>V<\/jats:italic><jats:sub>c<\/jats:sub>) and serum CT when compared with vehicle\u2010treated Orx rats. Analysis of trabecular microarchitecture of the tibia showed that administration of TAM significantly increased cancellous bone area, trabecular thickness and trabecular number, whereas trabecular separation was significantly decreased compared with vehicle\u2010treated Orx rats. Serum OC and urinary Ca<jats:sup>2+<\/jats:sup> concentrations were significantly lower in comparison with the control Orx group. These results indicate that in our rat model of androgen deficiency, TAM stimulated calcitonin\u2010producing thyroid C\u2010cells and increased trabecular bone mass.<\/jats:p>","DOI":"10.1111\/joa.12298","type":"journal-article","created":{"date-parts":[[2015,4,7]],"date-time":"2015-04-07T09:58:56Z","timestamp":1428400736000},"page":"489-496","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Tamoxifen stimulates calcitonin\u2010producing thyroid C\u2010cells and prevents trabecular bone loss in a rat model of androgen deficiency"],"prefix":"10.1111","volume":"226","author":[{"given":"Branko","family":"Filipovi\u0107","sequence":"first","affiliation":[{"name":"Institute for Biological Research \u2033Sini\u0161a Stankovi\u0107\u2033 University of Belgrade Belgrade Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Branka","family":"\u0160o\u0161i\u0107\u2010Jurjevi\u0107","sequence":"additional","affiliation":[{"name":"Institute for Biological Research \u2033Sini\u0161a Stankovi\u0107\u2033 University of Belgrade Belgrade Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Ajd\u017eanovi\u0107","sequence":"additional","affiliation":[{"name":"Institute for Biological Research \u2033Sini\u0161a Stankovi\u0107\u2033 University of Belgrade Belgrade Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jasmina","family":"\u017divanovi\u0107","sequence":"additional","affiliation":[{"name":"Institute for Biological Research \u2033Sini\u0161a Stankovi\u0107\u2033 University of Belgrade Belgrade Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esma","family":"Isenovi\u0107","sequence":"additional","affiliation":[{"name":"Vinca Institute of Nuclear Science Laboratory for Molecular Genetics and Radiobiology University of Belgrade Belgrade Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florina","family":"Popovska\u2010Per\u010dini\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine \u2033Ss Cyril and Methodius\u2033 University of Skopje Skopje FYR of Macedonia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Verica","family":"Milo\u0161evi\u0107","sequence":"additional","affiliation":[{"name":"Institute for Biological Research \u2033Sini\u0161a Stankovi\u0107\u2033 University of Belgrade Belgrade Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,4,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-002-0337-7"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19940701)74:1<74::AID-CNCR2820740113>3.0.CO;2-#"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-007-0379-6"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.86.5.2309"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-978618"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02405084"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711360104"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199506153322401"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000146936"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3388021"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.134.5.8156931"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-003-0578-4"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-007-0307-5"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.12013"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2004.tb02823.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.22.805"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000165166.36280.60"},{"key":"e_1_2_6_19_1","first-page":"4020","article-title":"Antitumor actions of keoxifene and tamoxifen in the N\u2010nitrosomethylurea\u2010induced rat mammary carcinoma model","volume":"47","author":"Gottardis MM","year":"1987","journal-title":"Cancer Res"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0029-7844(94)00383-O"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-118-6-2594"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecl.2005.07.009"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.12.5925"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.1.06264"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0026-0495(00)91533-6"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(97)00072-X"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005973529921"},{"key":"e_1_2_6_28_1","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1038\/sj.bjp.0707232","article-title":"Differential effects of selective oestrogen receptor modulators (SERMs) tamoxifen, ospemifene and raloxifene on human osteoclasts in vitro","volume":"15","author":"Michael H","year":"2007","journal-title":"Br J Pharmacol"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116134"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112584"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199811263392207"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650020617"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1996.14.1.78"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-9260(83)80408-5"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004180050225"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1210\/rp.57.1.317"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119701"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.4158\/EP.14.2.201"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.2147\/CIA.S820"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-122-3-1146"},{"key":"e_1_2_6_41_1","doi-asserted-by":"crossref","first-page":"2906","DOI":"10.1210\/endo.130.5.1572302","article-title":"Bone and mineral metabolism in aged male rats: short\u2010 and long\u2010term effects of androgen deficiency","volume":"130","author":"Vanderschueren D","year":"1992","journal-title":"Endocrinology"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00307.2002"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(86)90492-3"},{"key":"e_1_2_6_44_1","first-page":"2760","article-title":"Estrogen receptor and hormone responsiveness of medullary thyroid carcinoma cells in continuous culture","volume":"48","author":"Yang K","year":"1988","journal-title":"Cancer Res"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1260473"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(02)00688-9"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1385\/EP:14:2:159"}],"container-title":["Journal of Anatomy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjoa.12298","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjoa.12298","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/joa.12298","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T17:19:45Z","timestamp":1696267185000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/joa.12298"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,7]]},"references-count":46,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["10.1111\/joa.12298"],"URL":"https:\/\/doi.org\/10.1111\/joa.12298","archive":["Portico"],"relation":{},"ISSN":["0021-8782","1469-7580"],"issn-type":[{"type":"print","value":"0021-8782"},{"type":"electronic","value":"1469-7580"}],"subject":[],"published":{"date-parts":[[2015,4,7]]},"assertion":[{"value":"2015-02-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-04-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}