{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T04:34:06Z","timestamp":1790138046447,"version":"4.0.1"},"reference-count":65,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,11,11]],"date-time":"2015-11-11T00:00:00Z","timestamp":1447200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["11220110100889"],"award-info":[{"award-number":["11220110100889"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["2007\u20132202"],"award-info":[{"award-number":["2007\u20132202"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["2011\u20101895"],"award-info":[{"award-number":["2011\u20101895"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["2013\u2010404"],"award-info":[{"award-number":["2013\u2010404"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2012\/10000\u20105"],"award-info":[{"award-number":["2012\/10000\u20105"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2013\/50741\u20107"],"award-info":[{"award-number":["2013\/50741\u20107"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Serous (granular or venom) glands occur in the skin of almost all species of adult amphibians, and are thought to be the source of a great diversity of chemical compounds. Despite recent advances in their chemistry, odorous volatile substances are compounds that have received less attention, and until now no study has attempted to associate histological data with the presence of these molecules in amphibians, or in any other vertebrate. Given the recent identification of 40 different volatile compounds from the skin secretions of <jats:italic>H. pulchellus<\/jats:italic> (a treefrog species that releases a strong odour when handled), we examined the structure, ultrastructure, histochemistry, and distribution of skin glands of this species. Histological analysis from six body regions reveals the presence of two types of glands that differ in their distribution. Mucous glands are homogeneously distributed, whereas serous glands are more numerous in the scapular region. Ultrastructural results indicate that electron\u2010translucent vesicles observed within granules of serous glands are similar to those found in volatile\u2010producing glands from insects and also with lipid vesicles from different organisms. Association among lipids and volatiles is also evidenced from chemical results, which indicate that at least some of the volatile components in <jats:italic>H. pulchellus<\/jats:italic> probably originate within the metabolism of fatty acids or the mevalonate pathway. As odorous secretions are often considered to be secreted under stress situations, the release of glandular content was assessed after pharmacological treatments, epinephrine administrated <jats:italic>in\u00a0vivo<\/jats:italic> and on skin explants, and through surface electrical stimulation. Serous glands responded to all treatments, generally through an obvious contraction of myoepithelial cells that surround their secretory portion. No response was observed in mucous glands. Considering these morpho\u2010functional results, along with previous identification of volatiles from <jats:italic>H. pulchellus<\/jats:italic> and <jats:italic>H. riojanus<\/jats:italic> after electrical stimulation, we suggest that the electron\u2010translucent inclusions found within the granules of serous glands likely are the store sites of volatile compounds and\/or their precursors. Histochemical and glandular distribution analyses in five other species of frogs of the hylid tribe Cophomantini, revealed a high lipid content in all the species, whereas a heterogeneous distribution of serous glands is only observed in species of the <jats:italic>H. pulchellus<\/jats:italic> group. The distribution pattern of serous glands in members of this species group, and the odorous volatile secretions are probably related to defensive functions.<\/jats:p>","DOI":"10.1111\/joa.12413","type":"journal-article","created":{"date-parts":[[2015,11,11]],"date-time":"2015-11-11T06:58:52Z","timestamp":1447225132000},"page":"430-442","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Odorous secretions in anurans: morphological and functional assessment of serous glands as a source of\u00a0volatile compounds in the skin of the treefrog <i>Hypsiboas pulchellus<\/i> (Amphibia: Anura: Hylidae)"],"prefix":"10.1111","volume":"228","author":[{"given":"Andr\u00e9s E.","family":"Brunetti","sequence":"first","affiliation":[{"name":"Divisi\u00f3n Herpetolog\u00eda Museo Argentino de Ciencias Naturales \u2018Bernardino Rivadavia\u2019 \u2013 CONICET Buenos Aires Argentina"},{"name":"N\u00facleo de Pesquisa em Produtos Naturais e Sint\u00e9ticos (NPPNS) Departamento de F\u00edsica e Qu\u00edmica Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo Ribeir\u00e3o Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gladys N.","family":"Hermida","sequence":"additional","affiliation":[{"name":"Laboratorio Biolog\u00eda de Anfibios \u2013 Histolog\u00eda Animal Departamento de Biodiversidad y Biolog\u00eda Experimental Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires, Ciudad Universitaria Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana G.","family":"Iurman","sequence":"additional","affiliation":[{"name":"Laboratorio Biolog\u00eda de Anfibios \u2013 Histolog\u00eda Animal Departamento de Biodiversidad y Biolog\u00eda Experimental Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires, Ciudad Universitaria Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juli\u00e1n","family":"Faivovich","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n Herpetolog\u00eda Museo Argentino de Ciencias Naturales \u2018Bernardino Rivadavia\u2019 \u2013 CONICET Buenos Aires Argentina"},{"name":"Departamento de Biodiversidad y Biolog\u00eda Experimental Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires, Ciudad Universitaria Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,11,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/00222930701400954"},{"key":"e_1_2_8_3_1","first-page":"85","volume-title":"Theory and Practice of Histological Techniques","author":"Anderson G","year":"2002"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jzo.12044"},{"key":"e_1_2_8_5_1","volume-title":"Theory and Practice of Histological Techniques","author":"Bancroft JD","year":"2002"},{"key":"e_1_2_8_6_1","first-page":"73","article-title":"Ultrastructure of the myoepithelial cell of the cutaneous glands in several amphibian species","volume":"1","author":"Bani G","year":"1990","journal-title":"Biomed Res"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.01623"},{"key":"e_1_2_8_8_1","first-page":"129","article-title":"Los Hylidae de Punta Lara, Provincia de Buenos Aires. Observaciones sistem\u00e1ticas, ecol\u00f3gicas y an\u00e1lisis espectrogr\u00e1fico del canto","volume":"23","author":"Barrio A","year":"1962","journal-title":"Physis"},{"key":"e_1_2_8_9_1","first-page":"115","article-title":"La subspecies de Hyla pulchella Dumeril y Bibron (Anura, Hylidae)","volume":"25","author":"Barrio A","year":"1965","journal-title":"Physis"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0010-406X(69)90040-1"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2307\/1443948"},{"key":"e_1_2_8_12_1","first-page":"253","volume-title":"Reproductive Biology and Phylogeny of Anura","author":"Brizzi R","year":"2003"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.20056"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/bij.12406"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10886-015-0564-z"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.20407"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.1.9"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091770408"},{"key":"e_1_2_8_19_1","first-page":"179","article-title":"Merocrine secretion from serous cutaneous glands in Rana esculenta complex and Rana iberica","volume":"13","author":"Delfino G","year":"1996","journal-title":"Alytes"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/03946975.1998.10539370"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2005.11.002"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.64.3.724"},{"key":"e_1_2_8_23_1","first-page":"261","article-title":"New records of anuran predation by snakes of the genus Thamnodynastes Wagler, 1830 (Colubridae: Dipsadinae) in the Atlantic rainforest of southeastern Brazil","volume":"7","author":"Dorigo TA","year":"2014","journal-title":"Herpetol Notes"},{"key":"e_1_2_8_24_1","first-page":"178","volume-title":"Amphibian Biology, the Integument","author":"Erspamer V","year":"1994"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1096-0031.2009.00287.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1206\/3792.1"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1910(99)00162-6"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1467-8039(01)00027-5"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-00989-5_7"},{"key":"e_1_2_8_30_1","first-page":"291","article-title":"Observaciones sobre el comportamiento de algunos anfibios argentinos","volume":"14","author":"Gallardo JM","year":"1958","journal-title":"Cienc Invest"},{"key":"e_1_2_8_31_1","first-page":"49","article-title":"Observaciones biologicas sobre Hyla raddiana Fitz., de la provincia de Buenos Aires","volume":"17","author":"Gallardo JM","year":"1961","journal-title":"Cienc Invest"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2011.12.012"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)41992-3"},{"key":"e_1_2_8_34_1","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1016\/S0022-2275(20)34444-8","article-title":"The roles of Golgi and endoplasmic reticulum in the synthesis and assembly of lipoprotein lipids in rat hepatocytes","volume":"25","author":"Higgins JA","year":"1984","journal-title":"J Lipid Res"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402020203"},{"key":"e_1_2_8_36_1","volume-title":"Ranas y sapos del Uruguay: reconocimiento y aspectos biol\u00f3gicos","author":"Langone JA","year":"1994"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(93)90057-R"},{"key":"e_1_2_8_38_1","volume-title":"T\u00e9cnicas de Histolog\u00eda Animal","author":"Martoja R","year":"1970"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-6480(83)90133-8"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.10960"},{"key":"e_1_2_8_41_1","first-page":"628","article-title":"The coloration of acidic carbohydrates of bacteria and fungi in tissue sections with special reference to capsules of Cryptococcus neoformans Pneumococci and Staphylococci","volume":"32","author":"Mowry RW","year":"1956","journal-title":"Am J Pathol"},{"key":"e_1_2_8_42_1","first-page":"1","article-title":"Discovery of a defensively malodorous and nocturnal frog in the family Dendrobatidae: phylogenetic significance of a new genus and species from the Venezuelan Andes","volume":"3002","author":"Myers CW","year":"1991","journal-title":"Am Mus Novit"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(79)90029-6"},{"key":"e_1_2_8_44_1","first-page":"786","volume-title":"Histochemistry: Theorethical and Applied","author":"Pearse AGE","year":"1985"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201106592"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/np400131q"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.11021"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micron.2007.08.006"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(83)90037-X"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1139\/z99-248"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1021\/np4009317"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00226050"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1080\/02757540008037674"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1025686418909"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1163\/1570754041231587"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00990405"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00312154"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/bij.12167"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.2307\/1446304"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/0300-9629(95)98515-I"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1080\/03949370.2010.534321"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/1056-8719(92)90004-K"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004290050323"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1039\/b509854h"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7998.1974.tb04382.x"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402320103"}],"container-title":["Journal of Anatomy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjoa.12413","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjoa.12413","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/joa.12413","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T10:20:16Z","timestamp":1696242016000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/joa.12413"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,11]]},"references-count":65,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["10.1111\/joa.12413"],"URL":"https:\/\/doi.org\/10.1111\/joa.12413","archive":["Portico"],"relation":{},"ISSN":["0021-8782","1469-7580"],"issn-type":[{"value":"0021-8782","type":"print"},{"value":"1469-7580","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11,11]]},"assertion":[{"value":"2015-10-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-11-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}