{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,19]],"date-time":"2023-09-19T06:15:12Z","timestamp":1695104112864},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T00:00:00Z","timestamp":1479254400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R01DC009236"],"award-info":[{"award-number":["R01DC009236"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cartilage morphogenesis during endochondral ossification follows a progression of conserved developmental events. Cells are specified towards a prechondrogenic fate and subsequently undergo condensation followed by overt differentiation. Currently available molecular markers of prechondrogenic and condensing mesenchyme rely on common regulators of the chondrogenic program that are not specific to the tissue type or location. Therefore tissue\u2010specific condensations cannot be distinguished based on known molecular markers. Here, using the chick embryo model, we utilized lectin labeling on serial sections, demonstrating that differential labeling by peanut agglutinin (<jats:styled-content style=\"fixed-case\">PNA<\/jats:styled-content>) and <jats:italic>Sambucus nigra<\/jats:italic> agglutinin (<jats:styled-content style=\"fixed-case\">SNA<\/jats:styled-content>) successfully separates adjacently located condensations in the proximal second pharyngeal arch. <jats:styled-content style=\"fixed-case\">PNA<\/jats:styled-content> selectively labels chick middle ear columella and basal plate condensation, whereas <jats:styled-content style=\"fixed-case\">SNA<\/jats:styled-content> specifically marks extracolumella and the ventro\u2010lateral part of the otic capsule. We further extended our study to examine lectin\u2010binding properties of the different parts of the inner ear epithelium, neural tube and notochord. Our results show that <jats:styled-content style=\"fixed-case\">SNA<\/jats:styled-content> labels the auditory and vestibular hair cells of the inner ear, whereas <jats:styled-content style=\"fixed-case\">PNA<\/jats:styled-content> specifically recognizes the statoacoustic ganglion. <jats:styled-content style=\"fixed-case\">PNA<\/jats:styled-content> is also highly specific for the floor plate of the neural tube. Additionally, wheat germ agglutinin (<jats:styled-content style=\"fixed-case\">WGA<\/jats:styled-content>) labels the basement membrane of the notochord and is a marker of the apical\u2010basal polarity of the cochlear duct. Overall, this study indicates that selective lectin labeling is a promising approach to differentiate between contiguously located mesenchymal condensations and subregions of epithelia globally during development.<\/jats:p>","DOI":"10.1111\/joa.12565","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T19:45:53Z","timestamp":1479325553000},"page":"424-434","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Lectins selectively label cartilage condensations and the otic neuroepithelium within the embryonic chicken head"],"prefix":"10.1111","volume":"230","author":[{"given":"Poulomi","family":"Ray","sequence":"first","affiliation":[{"name":"Biological Sciences Clemson University Clemson SC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ami J.","family":"Hughes","sequence":"additional","affiliation":[{"name":"Biological Sciences Clemson University Clemson SC 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