{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T19:32:29Z","timestamp":1769628749531,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008225","name":"Midwestern State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008225","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001583","name":"Diabetes Action Research and Education Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001583","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>New methods on optical clearing provide a valuable alternative to traditional physical section histology. Optical clearing allows investigation of relatively large tissue samples at histological resolution while maintaining the three\u2010dimensional architecture of the intact system. There is significant potential for applying optical clearing to gastrointestinal tissues. In particular, intestinal crypts contain high concentrations of stem cells, making these structures especially important for research on cellular proliferation in the intestinal epithelium. The objective of our study is to demonstrate an optical clearing method that is easy to implement and is compatible with mitotic fluorescent labeling. The optical clearing method we present utilizes a Triton\/DMSO delipidization step followed by refractive index matching, rendering the tissue nearly transparent. We use EdU click chemistry to fluorescently label mitotic cell nuclei. Our results demonstrate successful clearing of jejunal samples with readily visible EdU staining by means of confocal microscopy. Three\u2010dimensional reconstruction of labeled samples reveals preservation of intestinal cytoarchitecture including muscular, submucosal, and mucosal layers. Additionally, the morphology of intestinal crypts and individual EdU\u2010positive mitotic nuclei are visible in sharp detail within their intact three\u2010dimensional organization. In summary, we present an optical clearing method that is easy to implement and has the potential to provide more accurate assessment of cellular proliferation within the gastrointestinal tract in both healthy and disease states.<\/jats:p>","DOI":"10.1111\/joa.12711","type":"journal-article","created":{"date-parts":[[2017,10,2]],"date-time":"2017-10-02T04:16:36Z","timestamp":1506917796000},"page":"152-157","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Optical clearing of small intestine for three\u2010dimensional visualization of cellular proliferation within crypts"],"prefix":"10.1111","volume":"232","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8964-4464","authenticated-orcid":false,"given":"Jason A.","family":"Kaufman","sequence":"first","affiliation":[{"name":"Department of Anatomy Arizona College of Osteopathic Medicine Midwestern University Glendale AZ 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