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In fish, such as the marine teleost gilthead seabream (<jats:italic>Sparus aurata<\/jats:italic>), the control of fluid homeostasis in the spermatic duct seems also to be crucial for male fertility, but no information exists on the expression and distribution of aquaporins. In this study, reverse transcriptase\u2010polymerase chain reaction and immunoblotting analyses, employing available and newly raised paralog\u2010specific antibodies for seabream aquaporins, indicate that up to nine functional aquaporins, Aqp0a, \u20101aa, \u20101ab, \u20103a, \u20104a, \u20107, \u20108bb, \u20109b and \u201010b, are expressed in the spermatic duct. Immunolocalization of the channels in the resting spermatic duct reveals that Aqp0a, \u20101aa, \u20104a, \u20107 and \u201010b are expressed in the monolayered luminal epithelium, Aqp8b and \u20109b in smooth muscle fibers, and Aqp1ab and \u20103a in different interstitial lamina cells. In the epithelial cells, Aqp0a and \u20101aa are localized in the short apical microvilli, and Aqp4a and \u201010b show apical and basolateral staining, whereas Aqp7 is solely detected in vesicular compartments. Upon spermiation, an elongation of the epithelial cells sterocilia, as well as the folding of the epithelium, is observed. At this stage, single\u2010 and double\u2010immunostaining, using two aquaporin paralogs or the Na<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>\u2010<jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>ase membrane marker, indicate that Aqp1ab, \u20103a, \u20107, \u20108bb and \u20109b staining remains unchanged, whereas in epithelial cells Aqp1aa translation is supressed, Aqp4a internalizes, and Aqp0a and \u201010b accumulate in the apical, lateral and basal plasma membrane. These findings uncover a cell type\u2010 and region\u2010specific distribution of multiple aquaporins in the piscine spermatic duct, which shares conserved features of the mammalian system. The data therefore suggest that aquaporins may play different roles in the regulation of fluid homeostasis and sperm maturation in the male reproductive tract of fish.<\/jats:p>","DOI":"10.1111\/joa.12829","type":"journal-article","created":{"date-parts":[[2018,5,28]],"date-time":"2018-05-28T02:50:36Z","timestamp":1527475836000},"page":"177-192","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The cellular localization and redistribution of multiple aquaporin paralogs in the spermatic duct epithelium of a maturing marine teleost"],"prefix":"10.1111","volume":"233","author":[{"given":"Fran\u00e7ois","family":"Chauvign\u00e9","sequence":"first","affiliation":[{"name":"Group of Comparative Molecular Physiology IRTA\u2010Institut de Ci\u00e8ncies del Mar Consejo Superior de Investigaciones Cient\u00edficas (CSIC) Barcelona 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