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Chemocommunication is mediated by two distinct organs located in the nasal cavity, the main olfactory epithelium and the vomeronasal organ (<jats:styled-content style=\"fixed-case\">VNO<\/jats:styled-content>). However, there is a lack of knowledge about the vomeronasal system in rabbits. To understand the role of this system, an exhaustive anatomical and histological study of the rabbit <jats:styled-content style=\"fixed-case\">VNO<\/jats:styled-content> was performed. The rabbit <jats:styled-content style=\"fixed-case\">VNO<\/jats:styled-content> was studied macroscopically by light microscopy, and by histochemical and immunohistochemical techniques. We employed specific histological staining techniques (periodic acid\u2010Schiff, Alcian blue, Gallego's trichrome), confocal autofluorescence, histochemical labelling with the lectin <jats:italic>Ulex europaeus<\/jats:italic> agglutinin (<jats:styled-content style=\"fixed-case\">UEA<\/jats:styled-content>\u2010I), and immunohistochemical studies of the expression of the G\u03b1i2 and G\u03b1o proteins and olfactory marker protein. The opening of the vomeronasal duct into the nasal cavity and its indirect communication with the oral cavity through a functional nasopalatine duct was demonstrated by classical dissection and microdissection. In a series of transverse histological sections, special attention was paid to the general distribution of the various soft\u2010tissue components of this organ (duct, glands, connective tissue, blood vessels and nerves) and to the nature of the capsule of the organ. Among the main morphological features that distinguish the rabbit <jats:styled-content style=\"fixed-case\">VNO<\/jats:styled-content>, the presence of a double envelope, which is bony externally and cartilaginous internally, and highly developed venous sinuses stand out. This observation indicates the crucial role played in this species by the pumping mechanism that introduces chemical signals into the vomeronasal duct. The functional properties of the organ are also confirmed by the presence of a well\u2010developed neuroepithelium and profuse glandular tissue that is positive for neutral mucopolysaccharides. The role of glycoconjugates was assessed by the identification of the \u03b11\u20102 fucose glycan system in the neuroepithelium of the <jats:styled-content style=\"fixed-case\">VNO<\/jats:styled-content> employing <jats:styled-content style=\"fixed-case\">UEA<\/jats:styled-content>\u2010I lectin. The pattern of labelling, which was concentrated around the commissures of the sensory epithelium and more diffuse in the central segments, is different from that found in most mammals studied. According to the expression of G\u2010proteins, two pathways have been described in the <jats:styled-content style=\"fixed-case\">VNO<\/jats:styled-content>s of mammals: neuroreceptor cells expressing the G\u03b1i2 protein (associated with vomeronasal receptor type 1); and cells expressing G\u03b1o (associated with vomeronasal receptor type 2). The latter pathway is absent in most mammals studied. The expression of both G\u2010protein families in the rabbit <jats:styled-content style=\"fixed-case\">VNO<\/jats:styled-content> places Lagomorpha together with rodents and insectivores in a small group of mammals belonging to the two\u2010path model. These findings support the notion that the rabbit possesses a highly developed <jats:styled-content style=\"fixed-case\">VNO<\/jats:styled-content>, with many specific morphological features, which highlights the significance of chemocommunication in this species.<\/jats:p>","DOI":"10.1111\/joa.12884","type":"journal-article","created":{"date-parts":[[2018,9,26]],"date-time":"2018-09-26T02:05:35Z","timestamp":1537927535000},"page":"814-827","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Morphological and immunohistochemical study of the rabbit vomeronasal organ"],"prefix":"10.1111","volume":"233","author":[{"given":"Paula R.","family":"Villamayor","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Department of Anatomy, Animal Production and Clinical Veterinary Sciences University of Santiago de Compostela Lugo Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Manuel","family":"Cifuentes","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Department of Anatomy, Animal Production and Clinical Veterinary Sciences University of Santiago de Compostela Lugo Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Fdz.\u2010de\u2010Troconiz","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Department of Anatomy, Animal Production and Clinical Veterinary Sciences University of Santiago de Compostela Lugo Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9891-4817","authenticated-orcid":false,"given":"Pablo","family":"Sanchez\u2010Quinteiro","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Department of Anatomy, Animal Production and Clinical Veterinary Sciences University of Santiago de Compostela Lugo Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(78)90616-9"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2014.00063"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2005.10.002"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2009.01147.x"},{"key":"e_1_2_7_6_1","unstructured":"BouvierAC JacquinetC(2008)Pheromone in rabbits. 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