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Combined retrograde tracing and immunofluorescence methods were used. Injections of the neuronal tracer Fast Blue (<jats:styled-content style=\"fixed-case\">FB<\/jats:styled-content>) into the right Ca<jats:styled-content style=\"fixed-case\">MG<\/jats:styled-content>revealed no retrogradely labelled (<jats:styled-content style=\"fixed-case\">FB<\/jats:styled-content>\u2010positive;<jats:styled-content style=\"fixed-case\">FB<\/jats:styled-content><jats:sup>+<\/jats:sup>) nerve cells in the intramural ganglia; however, many<jats:styled-content style=\"fixed-case\">FB<\/jats:styled-content><jats:sup>+<\/jats:sup>neurons were found in the spinal cord sympathetic nuclei. Double\u2010labelling immunohistochemistry revealed that nearly all (99.4\u00a0\u00b1\u00a00.6%) retrogradely labelled neurons were cholinergic (choline acetyltransferase\u2010positive;<jats:styled-content style=\"fixed-case\">ChAT<\/jats:styled-content><jats:sup>+<\/jats:sup>) in nature. Many<jats:styled-content style=\"fixed-case\">FB<\/jats:styled-content><jats:sup>+<\/jats:sup>\/<jats:styled-content style=\"fixed-case\">ChAT<\/jats:styled-content><jats:sup>+<\/jats:sup>perikarya stained positive for vesicular acetylcholine transporter (63.11\u00a0\u00b1\u00a05.34%), neuronal nitric oxide synthase (53.48\u00a0\u00b1\u00a09.62%) or cocaine\u2010 and amphetamine\u2010regulated transcript peptide (41.13\u00a0\u00b1\u00a04.77%). A small number of the retrogradely labelled cells revealed immunoreactivity for calcitonin gene\u2010related peptide (7.60\u00a0\u00b1\u00a01.34%) or pituitary adenylate cyclase\u2010activating polypeptide (4.57\u00a0\u00b1\u00a01.43%). The present study provides the first detailed information on the arrangement and chemical features of preganglionic neurons projecting to the porcine Ca<jats:styled-content style=\"fixed-case\">MG<\/jats:styled-content>and, importantly, strong evidence suggesting the absence of viscerofugal projections from the<jats:styled-content style=\"fixed-case\">UBT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">IG<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/joa.12916","type":"journal-article","created":{"date-parts":[[2018,11,23]],"date-time":"2018-11-23T10:26:10Z","timestamp":1542968770000},"page":"263-273","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A study on preganglionic connections and possible viscerofugal projections from urinary bladder intramural ganglia to the caudal mesenteric ganglion in the pig"],"prefix":"10.1111","volume":"234","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2998-9468","authenticated-orcid":false,"given":"Ewa","family":"Lepiarczyk","sequence":"first","affiliation":[{"name":"Department of Human Physiology School Of Medicine University of Warmia and Mazury in Olsztyn Olsztyn Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Bossowska","sequence":"additional","affiliation":[{"name":"Department of Human Physiology School Of Medicine University of Warmia and Mazury in Olsztyn Olsztyn Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Skowro\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Human Physiology School Of Medicine University of Warmia and Mazury in Olsztyn Olsztyn Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariusz","family":"Majewski","sequence":"additional","affiliation":[{"name":"Department of Human Physiology School Of Medicine University of Warmia and Mazury in Olsztyn Olsztyn Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,11,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1838(94)00150-I"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.23808"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.1070290203"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.201300099"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4695(19980905)36:3<325::AID-NEU2>3.0.CO;2-Y"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-19-07353.2000"},{"key":"e_1_2_8_8_1","first-page":"367","article-title":"Distribution of primary afferent neurons associated with the porcine inferior mesenteric ganglion (IMG)","volume":"40","author":"Bossowska A","year":"2002","journal-title":"Folia Histochem Cytobiol"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.2478\/v10181-012-0053-z"},{"key":"e_1_2_8_10_1","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1093\/oso\/9780195051063.003.0004","volume-title":"Central Regulation of Autonomic Function","author":"Cabot JB","year":"1990"},{"key":"e_1_2_8_11_1","first-page":"635","article-title":"Co\u2010localisation of NOS\u2010 and ChAT\u2010immunoreactivity in the spinal autonomic nuclei of the pig. 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