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This phospholipid functions in membrane trafficking, as an anchor for actin cytoskeletons and as a regulator of intramembranous channels\/transporters. It is also a precursor of such second messengers as diacylglycerol, inositol triphosphate and phosphatidylinositol (3,4,5)\u2010triphosphate. In the present study, the expression and localization of endogenous PIP5Ks were examined in the three major salivary glands of young adult mice <jats:italic>in\u00a0situ<\/jats:italic>. In western blotting of normal control glands, immunoreactive bands for individual PIP5Ks were detectable, with the highest density in the parotid gland and the weakest density in the submandibular gland. In immuno\u2010light microscopy under non\u2010stimulated condition, weak immunoreactivity for PIP5K\u03b1 was confined to the apical plasmalemma in parotid, but not sublingual or submandibular, acinar cells. Immunoreactivity for PIP5K\u03b2 was weak to moderate and confined to ductal cells but not acinar cells, whereas that for PIP5K\u03b3 was selectively and intensely detected in myoepithelial cells but not acinar cells, and it was weak in ductal cells in the three glands. In western blot of the parotid gland stimulated by isoproterenol, a \u03b2\u2010adrenoceptor agonist, no changes were seen in the intensity of immunoreactive bands for any of the PIP5Ks. In contrast, in immuno\u2010light microscopy, the apical immunoreactivity for PIP5K\u03b1 in parotid acinar cells was transiently and distinctly increased after the stimulation. The increased immunoreactivity was ultrastructurally localized on most apical microvilli and along contiguous plasma membrane, where membranous invaginations of various shapes and small vesicles were frequently found. It was thus suggested that PIP5K\u03b1 is involved in post\u2010exocytotic membrane dynamics via microvillous membranes. The present finding further suggests that each of the three isoforms of PIP5K functions through its product PIP2 discretely in different cells of the glands to regulate saliva secretion.<\/jats:p>","DOI":"10.1111\/joa.12944","type":"journal-article","created":{"date-parts":[[2019,2,8]],"date-time":"2019-02-08T04:08:27Z","timestamp":1549598907000},"page":"502-514","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Localization of phosphatidylinositol 4\u2010phosphate 5\u2010kinase (PIP5K) \u03b1, \u03b2, \u03b3 in the three major salivary glands <i>in\u00a0situ<\/i> of mice and their response to \u03b2\u2010adrenoceptor stimulation"],"prefix":"10.1111","volume":"234","author":[{"given":"Suthankamon","family":"Khrongyut","sequence":"first","affiliation":[{"name":"Faculty of Medicine Department of Anatomy Electron Microscopy Unit Khon Kaen University Khon Kaen 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