{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T12:14:10Z","timestamp":1772799250831,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,4,4]],"date-time":"2019-04-04T00:00:00Z","timestamp":1554336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The hard palate and mid\u2010palatal suture are highly important for orthodontic treatment. In cases of transverse maxillary deficiency, palatal expansion is the treatment of choice. As nowadays a growing number of adult patients receive orthodontic treatment, an understanding of suture development throughout life is important to derive tailored orthodontic treatment techniques for each age group. Histological, histochemical and immunohistochemical stains (<jats:styled-content style=\"fixed-case\">haematoxylin &amp; eosin<\/jats:styled-content>, Azan, Movat pentachrome, Masson\u2013Goldner trichrome, Sirius Red, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> 31, osteopontin and <jats:styled-content style=\"fixed-case\">TRAP<\/jats:styled-content>) and histomorphometric analyses were re\u2010established to detect the structural conditions of the mid\u2010palatal suture in human cadavers of three different age groups (20\u201339, 40\u201359 and 60\u201380\u00a0years). The mid\u2010palatal suture of the selected age groups (total of <jats:italic>n<\/jats:italic>\u00a0=\u00a012; <jats:italic>n<\/jats:italic>\u00a0=\u00a04 in every group m\u00a0=\u00a0f) exhibited marked differences in sutural morphology and metabolism. A wide, interdigitated and well\u2010vascularized suture was observed in younger specimens compared with straighter and smaller sutures with fewer vessels and lower bone density in the 60\u201380\u00a0year group. The fibre composition within the sutural gap differed between the three age groups. Delicate fibres were found in the 20\u201339\u00a0year group, and a tightly interwoven 3D fibre\u2010network was observed in the 40\u201359\u00a0year group. Atrophy primarily characterized the fibres in the 60\u201380 year group. This evidence demonstrates differences between the evaluated groups. These results suggest that the staining methods used are suitable for the description and evaluation of the morphology and metabolism of mid\u2010palatal sutures. Further investigation is necessary to provide an in\u2010depth description of sutural maturation over a lifetime.<\/jats:p>","DOI":"10.1111\/joa.12985","type":"journal-article","created":{"date-parts":[[2019,4,4]],"date-time":"2019-04-04T07:41:38Z","timestamp":1554363698000},"page":"180-188","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Development of a novel histological and histomorphometric evaluation protocol for a standardized description of the mid\u2010palatal suture \u2013 An <i>ex vivo<\/i> study"],"prefix":"10.1111","volume":"235","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3563-687X","authenticated-orcid":false,"given":"Ines","family":"Willershausen","sequence":"first","affiliation":[{"name":"Frankfurt Orofacial Regenerative Medicine (FORM) Lab Department for Oral, Cranio\u2010Maxillofacial and Facial Plastic Surgery Medical Centre of the Goethe University Frankfurt\/Main Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina","family":"Erbe","sequence":"additional","affiliation":[{"name":"Department of Dentofacial Orthopaedics University Medical Center Johannes Gutenberg University Mainz Mainz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Al\u2010Maawi","sequence":"additional","affiliation":[{"name":"Frankfurt Orofacial Regenerative Medicine (FORM) Lab Department for Oral, Cranio\u2010Maxillofacial and Facial Plastic Surgery Medical Centre of the Goethe University Frankfurt\/Main Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Orlowska","sequence":"additional","affiliation":[{"name":"Frankfurt Orofacial Regenerative Medicine (FORM) Lab Department for Oral, Cranio\u2010Maxillofacial and Facial Plastic Surgery Medical Centre of the Goethe University Frankfurt\/Main Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heiner","family":"Wehrbein","sequence":"additional","affiliation":[{"name":"Department of Dentofacial Orthopaedics University Medical Center Johannes Gutenberg University Mainz Mainz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahram","family":"Ghanaati","sequence":"additional","affiliation":[{"name":"Frankfurt Orofacial Regenerative Medicine (FORM) Lab Department for Oral, Cranio\u2010Maxillofacial and Facial Plastic Surgery Medical Centre of the Goethe University Frankfurt\/Main Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,4]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"55","article-title":"Use of coffin\u2010springs","volume":"3","author":"Antoni K","year":"1972","journal-title":"Quintessence Int Dent 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