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In immunoblotting analysis, the expression of <jats:styled-content style=\"fixed-case\">PLD<\/jats:styled-content>1 was high at postnatal 4\u00a0weeks (P4W) and decreased at P8W, and it was at negligible levels at newborn stage (P0W) and postnatal 2\u00a0weeks (P2W). The expression of <jats:styled-content style=\"fixed-case\">PLD<\/jats:styled-content>1 was greater in females, and it was suppressed by administration of testosterone to female mice. In immuno\u2010light microscopy, immunoreactivity for <jats:styled-content style=\"fixed-case\">PLD<\/jats:styled-content>1 at P4W was moderate to intense, in the forms of dots and globules mainly in the apical domains of immature granular convoluted tubule (<jats:styled-content style=\"fixed-case\">GCT<\/jats:styled-content>)\u2010cells localized largely in the proximal portion of the female <jats:styled-content style=\"fixed-case\">GCT<\/jats:styled-content>. By P8W, it decreased in intensity and remained weak to moderate along the apical plasmalemma of cells throughout the course of the female <jats:styled-content style=\"fixed-case\">GCT<\/jats:styled-content>, whereas it was faint throughout the <jats:styled-content style=\"fixed-case\">GCT<\/jats:styled-content> of the male <jats:styled-content style=\"fixed-case\">SMG<\/jats:styled-content> at P4W and negligible at P8W. In immuno\u2010electron microscopy, immature <jats:styled-content style=\"fixed-case\">GCT<\/jats:styled-content>\u2010cells characterized by electron\u2010lucent granules were immunoreactive and the immunoreactive materials were deposited close to, but not within, those granules. Typical <jats:styled-content style=\"fixed-case\">GCT<\/jats:styled-content> cells, characterized by electron\u2010dense granules, were immunonegative. No significant immunoreaction for <jats:styled-content style=\"fixed-case\">PLD<\/jats:styled-content>1 was seen in acini of <jats:styled-content style=\"fixed-case\">SMG<\/jats:styled-content>s of either sex at any time point examined. It is suggested that <jats:styled-content style=\"fixed-case\">PLD<\/jats:styled-content>1 is involved in the signaling for secretion of immature <jats:styled-content style=\"fixed-case\">GCT<\/jats:styled-content> cells and influences differentiation of these cells, probably through their own secretory substances.<\/jats:p>","DOI":"10.1111\/joa.13073","type":"journal-article","created":{"date-parts":[[2019,8,12]],"date-time":"2019-08-12T06:20:58Z","timestamp":1565590858000},"page":"1125-1136","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Expression of endogenous phospholipase D1, localized in mouse submandibular gland, is greater in females and is suppressed by testosterone"],"prefix":"10.1111","volume":"235","author":[{"given":"Suthankamon","family":"Khrongyut","sequence":"first","affiliation":[{"name":"Electron Microscopy Unit Department of Anatomy Faculty of Medicine Khon Kaen University Khon Kaen 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