{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T21:52:16Z","timestamp":1783461136992,"version":"3.55.0"},"reference-count":58,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,5,6]],"date-time":"2020-05-06T00:00:00Z","timestamp":1588723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570993"],"award-info":[{"award-number":["81570993"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671005"],"award-info":[{"award-number":["81671005"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81974151"],"award-info":[{"award-number":["81974151"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tight junction (TJ) plays an important role in regulating paracellular fluid transport in salivary glands; however, little is known about the involvement of TJs in diabetes salivary glands. This study aimed to investigate the alterations of TJs and their possible contribution in diabetes\u2010induced hyposalivation. Here, we observed that the morphologies of submandibular glands (SMGs) were impaired, characterized by enlarged acini accumulation with giant secretory granules, which were significantly reduced in atrophic ducts in SMGs of db\/db mice, a spontaneous model of type\u20102 diabetes. However, the secretory granules were increased and scattered in the acini of diabetes parotid glands (PGs). Other ultrastructural damages including swollen mitochondria, expansive endoplasmic reticulum, and autophagosomes were observed in the diabetes group. The levels of TJ proteins including claudin\u20101 (Cldn1) and claudin\u20103 (Cldn3) were increased, whereas those of claudin\u20104 (Cldn4), occludin (Ocln), and zonula occludens\u20101 (ZO\u20101) were decreased in SMGs of db\/db mice. Higher Cldn1 and Cldn3 and lower claudin\u201010 (Cldn10) and Ocln levels were observed in PGs of diabetes mice. Taken together, the structures of SMGs and PGs were impaired in diabetes mice, and the disruption of TJ integrity in both SMGs and PGs may contribute to diabetes\u2010induced hyposalivation.<\/jats:p>","DOI":"10.1111\/joa.13203","type":"journal-article","created":{"date-parts":[[2020,5,6]],"date-time":"2020-05-06T12:00:40Z","timestamp":1588766440000},"page":"556-567","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Disruption of tight junctions contributes to hyposalivation of salivary glands in a mouse model of type 2 diabetes"],"prefix":"10.1111","volume":"237","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0126-7810","authenticated-orcid":false,"given":"Yan","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian\u2010Ying","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi\u2010Jin","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology Peking University School of Basic Medical Sciences Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, and Beijing Key Laboratory of Cardiovascular Receptors Research Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Cong","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology Peking University School of Basic Medical Sciences Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, and Beijing Key Laboratory of Cardiovascular Receptors Research Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology Peking University School of Basic Medical Sciences Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, and Beijing Key Laboratory of Cardiovascular Receptors Research Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li\u2010Ling","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology Peking University School of Basic Medical Sciences Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, and Beijing Key Laboratory of Cardiovascular Receptors Research Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang\u2010Yan","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruo\u2010Lan","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology Peking University School of Basic Medical Sciences Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, and Beijing Key Laboratory of Cardiovascular Receptors Research Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25736"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(86)90021-X"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.1070270105"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/21688370.2016.1162348"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.116971"},{"key":"e_1_2_8_7_1","volume-title":"Histology, Salivary Glands","author":"Brazen B.","year":"2020"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2019.11.008"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a029314"},{"key":"e_1_2_8_10_1","first-page":"309","article-title":"Structural differences between alcoholic and diabetic parotid sialosis","volume":"10","author":"Carda C.","year":"2005","journal-title":"Medicina Oral, Patolog\u00eda Oral y Cirug\u00eda Bucal"},{"key":"e_1_2_8_11_1","first-page":"E309","article-title":"Structural and functional salivary disorders in type 2 diabetic patients","volume":"11","author":"Carda C.","year":"2006","journal-title":"Medicina Oral, Patolog\u00eda Oral y Cirug\u00eda Bucal"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12712"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabres.2018.02.023"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2018.07.002"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.165878"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2012.12"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-199806000-00007"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2015.03.004"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-017-9716-5"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.27362"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197408)34:2<282::AID-CNCR2820340211>3.0.CO;2-W"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3363"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(85)90153-0"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.19082\/5244"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-018-9318-8"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2011.01824.x"},{"key":"e_1_2_8_27_1","first-page":"219","article-title":"Xerostomia and salivary levels of glucose and urea in patients with diabetes","volume":"33","author":"Ivanovski K.","year":"2012","journal-title":"Prilozi"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-019-0223-y"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.16-20412"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.2535\/ofaj.84.71"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/219843"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00020"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.00527"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.23255"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12238"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00458557"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345690480041101"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/9850398"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.17219\/acem\/37067"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12098"},{"key":"e_1_2_8_41_1","first-page":"e586","article-title":"Oral manifestations of diabetes mellitus. A systematic review","volume":"22","author":"Mauri\u2010Obradors E.","year":"2017","journal-title":"Med Oral Patol Oral Cir Bucal"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1369\/0022155414555145"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1369\/0022155416646089"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.4103\/jrms.JRMS_135_18"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2004.04.016"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/joor.12664"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0021-8782.2004.00332.x"},{"key":"e_1_2_8_48_1","first-page":"131","article-title":"Salivary glands in long\u2010term alloxan\u2010diabetic rats. a quantitative light and electron\u2010microscopic study","volume":"95","author":"Reuterving C.O.","year":"1987","journal-title":"Acta Pathol Microbiol Immunol Scand A"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.3889\/oamjms.2019.340"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19103208"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dsx.2019.07.037"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mju048"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-016-9709-9"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.13432"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.15-0589"},{"key":"e_1_2_8_56_1","first-page":"13","article-title":"Tight junctions and paracellular fluid and ion transport in salivary glands","volume":"16","author":"Zhang G.H.","year":"2013","journal-title":"The Chinese Journal of Dental Research"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034516634647"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27640"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.12833"}],"container-title":["Journal of Anatomy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjoa.13203","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/joa.13203","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/joa.13203","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/joa.13203","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T07:24:57Z","timestamp":1693898697000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/joa.13203"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,6]]},"references-count":58,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1111\/joa.13203"],"URL":"https:\/\/doi.org\/10.1111\/joa.13203","archive":["Portico"],"relation":{},"ISSN":["0021-8782","1469-7580"],"issn-type":[{"value":"0021-8782","type":"print"},{"value":"1469-7580","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,6]]},"assertion":[{"value":"2020-01-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}