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Post\u2010synaptic acetylcholine receptors (AChRs), which are found in high density in the muscle membrane, bind to acetylcholine released into the synaptic cleft of the NMJ, thereby enabling the conversion of motor action potentials to muscle contractions. NMJs have been studied for many years as a general model for synapse formation, development and function, and are known to be early sites of pathological changes in many neuromuscular diseases. However, information is limited on the diversity of NMJs in different muscles, how synaptic morphology changes during development, and the relevance of these parameters to neuropathology. Here, this crucial gap was addressed using a robust and standardised semi\u2010automated workflow called NMJ\u2010morph to quantify features of pre\u2010 and post\u2010synaptic NMJ architecture in an unbiased manner. Five wholemount muscles from wild\u2010type mice were dissected and compared at immature (post\u2010natal day, P7) and early adult (P31\u221232) timepoints. The inter\u2010muscular variability was greater in mature post\u2010synaptic AChR morphology than that of the pre\u2010synaptic motor neuron terminal. Moreover, the developing NMJ showed greater differences across muscles than the mature synapse, perhaps due to the observed distinctions in synaptic growth between muscles. Nevertheless, the amount of nerve to muscle contact was consistent, suggesting that pathological denervation can be reliably compared across different muscles in mouse models of neurodegeneration. Additionally, mature post\u2010synaptic endplate diameters correlated with fibre type, independently of muscle fibre diameter. Altogether, this work provides detailed information on healthy pre\u2010 and post\u2010synaptic NMJ morphology from five anatomically and functionally distinct mouse muscles, delivering useful reference data for future comparison with neuromuscular disease models.<\/jats:p>","DOI":"10.1111\/joa.13228","type":"journal-article","created":{"date-parts":[[2020,6,13]],"date-time":"2020-06-13T03:19:30Z","timestamp":1592018370000},"page":"603-617","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Morphological variability is greater at developing than mature mouse neuromuscular junctions"],"prefix":"10.1111","volume":"237","author":[{"given":"Aleksandra M.","family":"Mech","sequence":"first","affiliation":[{"name":"Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology University College London London 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