{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T09:41:58Z","timestamp":1768729318440,"version":"3.49.0"},"reference-count":52,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T00:00:00Z","timestamp":1611187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["#W1262\u2010B29"],"award-info":[{"award-number":["#W1262\u2010B29"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although laryngeal morphology often reflects adaptations for vocalization, the structural consequences of selection for particular aspects of vocal behavior remain poorly understood. In this study, we investigated the effects of increased ultrasonic calling in pups on the adult larynx morphology in selectively bred rat lines. Laryngeal morphology was assessed using multiple techniques: mineralized cartilage volumes were compared in 3D\u2010models derived from microCT scans, internal structure was compared using clearing and staining procedures combined with microscopy, cellular structure was compared using histology and microscopy, and element composition was assessed with scanning energy dispersive X\u2010ray spectroscopy. Our results show that adult rats from lines bred to produce ultrasonic calls at higher rates as pups have shorter vocal folds and a more mineralized thyroid cartilage compared to rats bred to produce ultrasonic calls at lower rates. The change in vocal fold length appears to account for differences in low\u2010frequency calls in these two rat lines. We suggest that the observed increases in mineralization of the thyroid cartilage in the high\u2010ultrasound lineage provide increased reinforcement of the laryngeal structure during ultrasonic call production. Our findings therefore demonstrate an effect of selection for vocal behavior on laryngeal morphology, with acoustic consequences.<\/jats:p>","DOI":"10.1111\/joa.13366","type":"journal-article","created":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T05:10:46Z","timestamp":1611292246000},"page":"1179-1190","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Selection on vocal output affects laryngeal morphology in rats"],"prefix":"10.1111","volume":"238","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7151-252X","authenticated-orcid":false,"given":"Raffaela","family":"Lesch","sequence":"first","affiliation":[{"name":"Department of Behavioral and Cognitive Biology University of Vienna Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Schwaha","sequence":"additional","affiliation":[{"name":"Department of Evolutionary Biology, Integrative Zoology University of Vienna Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Orozco","sequence":"additional","affiliation":[{"name":"Department of Psychology Williams College Williamstown MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret","family":"Shilling","sequence":"additional","affiliation":[{"name":"Department of Psychology Williams College Williamstown MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Brunelli","sequence":"additional","affiliation":[{"name":"Department of Psychiatry Columbia University New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myron","family":"Hofer","sequence":"additional","affiliation":[{"name":"Department of Psychiatry Columbia University New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel L.","family":"Bowling","sequence":"additional","affiliation":[{"name":"Department of Behavioral and Cognitive Biology University of Vienna Vienna Austria"},{"name":"Department of Psychiatry and Behavioral Sciences Stanford University School of Medicine Stanford CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Betty","family":"Zimmerberg","sequence":"additional","affiliation":[{"name":"Department of Psychology Williams College Williamstown MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William Tecumseh","family":"Fitch","sequence":"additional","affiliation":[{"name":"Department of Behavioral and Cognitive Biology University of Vienna Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4471-1437-6_7"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep41070"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000764"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-4328(01)00277-7"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10519-004-0858-3"},{"key":"e_1_2_9_7_1","volume-title":"Handbook of Mammalian Vocalization: An Integrative Neuroscience Approach","author":"Brudzynski S.M.","year":"2010"},{"key":"e_1_2_9_8_1","volume-title":"Handbook of Ultrasonic Vocalization: A Handbook into the Emotional Brain","author":"Brudzynski S.M.","year":"2018"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2302(199712)31:4<255::AID-DEV3>3.0.CO;2-Q"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10519-004-0855-6"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2007.04.014"},{"key":"e_1_2_9_12_1","first-page":"1","article-title":"Reinforcement of the larynx and trachea in echolocating and non\u2010echolocating bats\u2019","author":"Carter R.T.","year":"2020","journal-title":"Journal of Anatomy"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12739"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-92495-3"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2015.09.029"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9978"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-27721-9_10"},{"key":"e_1_2_9_18_1","first-page":"65","article-title":"Unpacking \u201cHonesty\u201d: Vertebrate Vocal Production and the Evolution of Acoustic Signals","author":"Fitch W.T.","year":"2006","journal-title":"Acoustic Communication"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-73906-9"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2006.00684.x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2011.01361.x"},{"key":"e_1_2_9_22_1","first-page":"197","article-title":"Larynx and vocalization of the Takin (Budorcas taxicolor Hodgson, 1850 \u2010 Mammalia, Bovidae)","volume":"239","author":"Frey R.","year":"2000","journal-title":"Zoologischer Anzeiger"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1078\/0044-5231-00086"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-11000-x"},{"key":"e_1_2_9_25_1","volume-title":"Bones and Cartilage: Developmental and Evolutionary Skeletal Biology","author":"Hall B.K.","year":"2015"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1984.tb05706.x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511525766"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1219712"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0194-5998(98)70378-X"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1121\/1.3462234"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027029"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/eth.13075"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2016.08.032"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1086\/283219"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anbehav.2014.06.011"},{"issue":"1","key":"e_1_2_9_36_1","first-page":"28","article-title":"Types and functions of ultrasonic vocalizations in laboratory rats and mice","volume":"46","author":"Portfors C.V.","year":"2007","journal-title":"Journal of the American Association for Laboratory Animal Science"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2011.04.024"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.170976"},{"key":"e_1_2_9_39_1","doi-asserted-by":"crossref","first-page":"2859","DOI":"10.1242\/jeb.202.20.2859","article-title":"Vocal tract length and acoustics of vocalization in the domestic dog (Canis familiaris)","volume":"202","author":"Riede T.","year":"1999","journal-title":"The Journal of experimental biology"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-989-5_9"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1096-3642.1975.tb00268.x"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-624X(75)90052-9"},{"issue":"88","key":"e_1_2_9_43_1","article-title":"The mechanism of ultrasonic vocalization in the rat","volume":"27","author":"Sanders I.","year":"2001","journal-title":"Society of Neuroscience Abstracts"},{"issue":"7","key":"e_1_2_9_44_1","first-page":"1","article-title":"Der Larynx der S\u00e4ugetiere","volume":"5","author":"Schneider R.","year":"1964","journal-title":"Handbuch der Zoologie. Berlin"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-9930-9_9"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7998.1967.tb04069.x"},{"key":"e_1_2_9_47_1","first-page":"373","volume-title":"Handbook of Mammalian Vocalization: An Integrative Neurosciennce Approach","author":"Shiba K.","year":"2009"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2018.06.018"},{"key":"e_1_2_9_49_1","volume-title":"Principles of Voice Production","author":"Titze I.R.","year":"1994"},{"key":"e_1_2_9_50_1","unstructured":"Titze I.R.(2006).The myoelastic aerodynamic theory of phonation in National Center for Voice & Speech."},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.044982"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.specom.2018.05.003"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-3057(94)90181-3"}],"container-title":["Journal of Anatomy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/joa.13366","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/joa.13366","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/joa.13366","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T14:06:41Z","timestamp":1693577201000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/joa.13366"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,21]]},"references-count":52,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,5]]}},"alternative-id":["10.1111\/joa.13366"],"URL":"https:\/\/doi.org\/10.1111\/joa.13366","archive":["Portico"],"relation":{},"ISSN":["0021-8782","1469-7580"],"issn-type":[{"value":"0021-8782","type":"print"},{"value":"1469-7580","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,21]]},"assertion":[{"value":"2020-08-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}