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Towards these goals, we assessed the capabilities of high\u2010resolution magnetic resonance imaging (MRI) and matrix\u2010assisted laser desorption\/ionisation\u2010mass spectrometry (MALDI\u2010MS) imaging of rarely reported paediatric and adult cadaveric larynges that contained pathologies. The donors were a 13\u2010month\u2010old male, a 10\u2010year\u2010old female with an infraglottic mucus retention cyst and a 74\u2010year\u2010old female with advanced polypoid degeneration and a mucus retention cyst. MR and molecular imaging data were corroborated using whole\u2010organ histology. Our MR protocols imaged the larynges at 45\u2013117\u00a0\u03bcm<jats:sup>2<\/jats:sup> in\u2010plane resolution and capably resolved microanatomic structures that have not been previously reported radiographically\u2014such as the vocal fold superficial lamina propria, vocal ligament and macula flavae; age\u2010related tissue features\u2014such as intramuscular fat deposition and cartilage ossification; and the lesions. Diffusion tensor imaging characterised differences in water diffusivity, primary tissue fibre orientation, and fractional anisotropy between the intrinsic laryngeal muscles, mucosae and lesions. MALDI\u2010MS imaging revealed peptide signatures and putative protein assignments for the polypoid degeneration lesion and the N\u2010glycan constituents of one mucus retention cyst. These imaging approaches have immediate application in experimental research and, with ongoing technology development, potential for future clinical application.<\/jats:p>","DOI":"10.1111\/joa.13451","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T10:33:30Z","timestamp":1621938810000},"page":"545-556","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["High\u2010resolution magnetic resonance and mass spectrometry imaging of the human larynx"],"prefix":"10.1111","volume":"239","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5071-6515","authenticated-orcid":false,"given":"Ayami Ohno","family":"Kishimoto","sequence":"first","affiliation":[{"name":"Department of Diagnostic Radiology Kyoto Katsura Hospital Kyoto 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