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Muscles of the distal leg, for instance the gastrocnemius and tibialis anterior, are commonly used in such studies with mice and rats. However, thin and flat muscles, which can be dissected, processed and imaged without major disruption to muscle fibres and nerve\u2010muscle contacts, are more suitable for accurate and detailed analyses of the peripheral motor nervous system. One such wholemount muscle is the predominantly fast twitch epitrochleoanconeus (ETA), which is located in the upper forelimb, innervated by the radial nerve, and contains relatively large and uniformly flat neuromuscular junctions (NMJs). To facilitate incorporation of the ETA into the experimental toolkit of the neuromuscular disease field, here, we describe a simple method for its rapid isolation (&lt;5\u00a0min), supported by high\u2010resolution videos and step\u2010by\u2010step images. Furthermore, we outline how the ETA can be imaged in live, anaesthetised mice, to enable examination of dynamic cellular processes occurring at the NMJ and within intramuscular axons, including transport of organelles, such as mitochondria and signalling endosomes. Finally, we present reference data on wild\u2010type ETA fibre\u2010type composition in young adult, male C57BL6\/J mice. Comparative neuroanatomical studies of different muscles in rodent models of disease can generate critical insights into pathogenesis and pathology; dissection of the wholemount ETA provides the possibility to diversify the repertoire of muscles analysed for this endeavour.<\/jats:p>","DOI":"10.1111\/joa.13478","type":"journal-article","created":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T04:04:01Z","timestamp":1623643441000},"page":"1108-1119","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Dissection, in vivo imaging and analysis of the mouse epitrochleoanconeus muscle"],"prefix":"10.1111","volume":"241","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4250-7216","authenticated-orcid":false,"given":"David","family":"Villarroel\u2010Campos","sequence":"first","affiliation":[{"name":"Department of Neuromuscular Diseases UCL Queen Square Institute of Neurology University College London  London 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