{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T17:51:56Z","timestamp":1790445116420,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T00:00:00Z","timestamp":1637625600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mast cells, known as pro\u2010inflammatory effector cells, are immunocytes present in the meninges and may be involved in the pathophysiology of migraine. This study aims to evaluate the histomorphometric parameters of mast cells located in the convexity of the human intracranial dura mater. For this, samples of intracranial dura mater from eight human fresh cadavers were collected between 8\u2010 and 24\u2010h post\u2010mortem. The whole samples were fixed and, subsequently, two fragments of 1.5\u00a0cm\u00b2 each were cut from four different areas of the dura mater convexity, containing a segment of the middle meningeal artery, totaling 64 fragments. After histological processing, the fragments were submitted to microtomy (5 and 10\u00a0\u00b5m), stained with toluidine blue (0.1%), or immunohistochemically labeled for tryptase, and analyzed using optical microscopy. The following histomorphometric parameters were evaluated: distance from mast cells to vessels, the density of mast cells, and percentage of mast cells with degranulation. Histomorphometric analyzes showed a higher density of mast cells in the vicinity of blood vessels (arterial and venous), with distances around 0\u2013150\u00a0\u00b5m. A greater number of mast cells was detected near venous vessels in the periosteal layer (17.0\u00a0\u00b1\u00a010.1\u00a0cells\/mm\u00b2) than in the meningeal layer (14.1\u00a0\u00b1\u00a07.0\u00a0cells\/mm\u00b2) (<jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.05). Mast cells from the region close to the superior sagittal sinus were found in greater quantity close to the venous vessels (16.7\u00a0\u00b1\u00a010.1\u00a0cells\/mm\u00b2) than to the arterial vessels (11.2\u00a0\u00b1\u00a07.5\u00a0cells\/mm\u00b2) (<jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.05). In short, in the convexity of the human intracranial dura mater, mast cells are located close to blood vessels, with a greater number of cells next to the venous vessels of the periosteal layer and in the proximal region of the superior sagittal sinus.<\/jats:p>","DOI":"10.1111\/joa.13585","type":"journal-article","created":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T05:25:10Z","timestamp":1637731510000},"page":"724-734","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Histomorphometry of mast cells in the convexity of human intracranial dura mater"],"prefix":"10.1111","volume":"240","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9895-5654","authenticated-orcid":false,"given":"Emanuela P.","family":"Rosas","sequence":"first","affiliation":[{"name":"Postgraduate Program in Biology applied to Health (PPGBAS) Keizo Asami Immunopathology Laboratory (LIKA) Federal University of Pernambuco (UFPE)  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1027-7022","authenticated-orcid":false,"given":"Silvania T.","family":"Paz","sequence":"additional","affiliation":[{"name":"Northeast Biotechnology Network (RENORBIO) UFPE  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1180-6689","authenticated-orcid":false,"given":"Raisa F.","family":"Costa","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biological Sciences (PPGCB) UFPE  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1066-8990","authenticated-orcid":false,"given":"Amanda A.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Neurosurgery Unit Federal University of Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5680-0902","authenticated-orcid":false,"given":"Romildo L.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Hospital das Cl\u00ednicas Pathological Anatomy Sector UFPE  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3264-2625","authenticated-orcid":false,"given":"Ana P. F.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biology applied to Health (PPGBAS) Keizo Asami Immunopathology Laboratory (LIKA) Federal University of Pernambuco (UFPE)  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4271-4098","authenticated-orcid":false,"given":"Sabrina R. S.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Animal Bioscience (PPGBA) Federal Rural University of Pernambuco (UFRPE)  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9517-1416","authenticated-orcid":false,"given":"Paloma L.","family":"de Medeiros","sequence":"additional","affiliation":[{"name":"Northeast Biotechnology Network (RENORBIO) UFPE  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7714-0608","authenticated-orcid":false,"given":"Manuela F. L.","family":"de Freitas","sequence":"additional","affiliation":[{"name":"Department of Anatomy UFPE  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0678-3782","authenticated-orcid":false,"given":"Marcelo M.","family":"Valen\u00e7a","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biology applied to Health (PPGBAS) Keizo Asami Immunopathology Laboratory (LIKA) Federal University of Pernambuco (UFPE)  Recife Brazil"},{"name":"Postgraduate Program in Biological Sciences (PPGCB) UFPE  Recife Brazil"},{"name":"Neurosurgery Unit Federal University of Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00309843"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.1085"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/0333102412454947"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1468-2982.1991.1104165.x"},{"issue":"1","key":"e_1_2_10_6_1","first-page":"259","article-title":"The mast cell: an active participant or an innocent bystander?","volume":"19","author":"Crivellato E.","year":"2004","journal-title":"Histology and Histopathology"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-185X.2009.00105.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(96)00488-5"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01252276"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00217397"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1468\u20102982.1981.0104175.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006\u20108993(82)90290\u20106"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpain.2013.03.010"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1212\/wnl.10.6.555"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.1074"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199301283280408"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)01344-3"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3109\/09513590.2014.911280"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092300103"},{"key":"e_1_2_10_20_1","first-page":"5","article-title":"Multiplicity of cerebrospinal fluid functions: new challenges in health and disease","volume":"14","author":"Johanson C.E.","year":"2008","journal-title":"Cerebrospinal Fluid Research"},{"key":"e_1_2_10_21_1","first-page":"212","volume-title":"Histologia b\u00e1sica: texto e atlas","author":"Junqueira L.C.","year":"2017"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2013.02.006"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-015-1790-z"},{"issue":"1","key":"e_1_2_10_24_1","first-page":"1","article-title":"Neuroinflammation induces neurodegeneration","volume":"1","author":"Kempuraj D.","year":"2016","journal-title":"Journal of Neurology, Neurosurgery and Spine"},{"issue":"2","key":"e_1_2_10_25_1","first-page":"65","article-title":"Significance of conversation between mast cells and nerves","volume":"1","author":"Kleij H.P.","year":"2005","journal-title":"Allergy, Asthma, and Clinical Immunology"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-07-358432"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.12659"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2007.03.012"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1177\/0333102418771350"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0b013e3282fd185e"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.07-12-04129.1987"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902230105"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19990322)405:4<553::AID-CNE8>3.0.CO;2-6"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00188214"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23911"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(79)92692-8"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.06-08-02200.1986"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1468-2982.1997.1703166.x"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464\u2010410x.1995.tb07303.x"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2015.11.022"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20030481"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(99)01855-7"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.npep.2017.04.008"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003574"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-2982.2007.01288.x"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.01293.2005"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20106"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306\u20104522(93)90030\u2010j"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2004.11.006"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.136.12.7588332"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2015.03.086"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00381\u2010012\u20101699\u20107"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1159\/000236217"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4179(90)90114-E"}],"container-title":["Journal of Anatomy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/joa.13585","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/joa.13585","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/joa.13585","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,27]],"date-time":"2023-08-27T04:45:37Z","timestamp":1693111537000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/joa.13585"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,23]]},"references-count":53,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["10.1111\/joa.13585"],"URL":"https:\/\/doi.org\/10.1111\/joa.13585","archive":["Portico"],"relation":{},"ISSN":["0021-8782","1469-7580"],"issn-type":[{"value":"0021-8782","type":"print"},{"value":"1469-7580","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,23]]},"assertion":[{"value":"2021-05-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}