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Tyramide signal amplification (TSA) is a powerful method used to improve the detection sensitivity of immunohistochemistry. RNAScope is a novel commercially available\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization assay for the detection of RNA expression. In this work, we describe the use of TSA and RNAScope\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization as extremely sensitive and specific methods for the evaluation of protein and RNA expression in formaldehyde\u2010fixed paraffin\u2010embedded human fetal brain sections. These two techniques, when properly optimized, were highly compatible with routine formaldehyde\u2010fixed paraffin\u2010embedded tissue that preserves the best morphological characteristics of delicate fetal brain samples, enabling an unparalleled ability to simultaneously visualize the expression of multiple protein and mRNA of genes that are sparsely expressed in the human fetal telencephalon.\n                  <\/jats:p>","DOI":"10.1111\/joa.13644","type":"journal-article","created":{"date-parts":[[2022,2,27]],"date-time":"2022-02-27T21:13:35Z","timestamp":1645996415000},"page":"33-41","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Tyramide signal amplification coupled with multiple immunolabeling and\n                    <scp>RNAScope<\/scp>\n                    <i>in situ<\/i>\n                    hybridization in formaldehyde\u2010fixed paraffin\u2010embedded human fetal brain"],"prefix":"10.1111","volume":"241","author":[{"given":"Ayman","family":"Alzu'bi","sequence":"first","affiliation":[{"name":"Biosciences Institute Newcastle University  Newcastle upon Tyne UK"},{"name":"Department of Basic Medical Sciences Yarmouk University  Irbid Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niveditha","family":"Sankar","sequence":"additional","affiliation":[{"name":"Biosciences Institute Newcastle University  Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moira","family":"Crosier","sequence":"additional","affiliation":[{"name":"Biosciences Institute Newcastle University  Newcastle upon Tyne UK"},{"name":"Human Developmental Biology Resource Newcastle University  Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet","family":"Kerwin","sequence":"additional","affiliation":[{"name":"Biosciences Institute Newcastle University  Newcastle upon Tyne 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