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Given the difficult access to fresh anatomical specimens, the constant renovation of samples for research and educational purposes is unsustainable, beyond the ethical issues involved. The standard technique for preserving specimens involves fixation and later immersion in formaldehyde, which enables a good result, but also presents elevated carcinogenic potential. Therefore, safe and efficient preservation methods are mandatory for anatomical practices and investigations. An accessible and inexpensive alternative for specimen preservation is cryodehydration. Cryodehydrated specimens can be kept dry, with no final odor, reducing the use and exposure to formaldehyde. The objective of this study was to propose a simplified step\u2010by\u2010step cryodehydration protocol to obtain high\u2010quality anatomical specimens. Through consecutive freezing and thawing cycles, cryodehydration caused a weight reduction of 60%\u201370% and allowed anatomical preservation, while maintaining the main morphological aspects of cavitary and parenchymatous organs, muscles, or even full\u2010body sections. The final specimens presented high durability and can be maintained for decades, preserving all relevant anatomical features.<\/jats:p>","DOI":"10.1111\/joa.13669","type":"journal-article","created":{"date-parts":[[2022,4,16]],"date-time":"2022-04-16T04:45:27Z","timestamp":1650084327000},"page":"545-551","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Cryodehydration protocol to obtain high\u2010quality permanent anatomical material"],"prefix":"10.1111","volume":"241","author":[{"given":"Leandro Luis","family":"Martins","sequence":"first","affiliation":[{"name":"Department of Anatomy State University of Londrina  Londrina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3143-4093","authenticated-orcid":false,"given":"Marna Eliana","family":"Sakalem","sequence":"additional","affiliation":[{"name":"Department of Anatomy State University of Londrina  Londrina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11055\u2010010\u20109371\u20108"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/rej.2014.1656"},{"key":"e_1_2_8_4_1","unstructured":"Bragatto L. 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