{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T12:49:53Z","timestamp":1780490993775,"version":"3.54.1"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,8,19]],"date-time":"2022-08-19T00:00:00Z","timestamp":1660867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP21K07772"],"award-info":[{"award-number":["JP21K07772"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20K22736"],"award-info":[{"award-number":["20K22736"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005683","name":"Kyoto University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005683","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rapid shelf elevation and contact of the secondary palate and fusion reportedly occur due to a growth\u2010related equilibrium change in the structures within the oro\u2010nasal cavity. This study aimed to quantitatively evaluate complex three\u2010dimensional morphological changes and their effects on rapid movements, such as shelf elevation and contact, and fusion. Morphological changes during secondary palate formation were analyzed using high\u2010resolution digitalized imaging data (phase\u2010contrast X\u2010ray computed tomography and magnetic resonance images) obtained from 22 human embryonic and fetal samples. The three\u2010dimensional images of the oro\u2010nasal structures, including the maxilla, palate, pterygoid hamulus, tongue, Meckel's cartilage, nasal cavity, pharyngeal cavity, and nasal septum, were reconstructed manually. The palatal shelves were not elevated in all the samples at Carnegie stage (CS)21 and CS22 and in three samples at CS23. In contrast, the palatal shelves were elevated but not in contact in one sample at CS23. Further, the palatal shelves were elevated and fused in the remaining four samples at CS23 and all three samples from the early fetal period. For each sample, 70 landmarks were subjected to Procrustes and principal component (PC) analysis. PC\u20101 accounted for 67.4% of the extracted gross changes before and after shelf elevations. Notably, the PC\u20101 values of the negative and positive value groups differed significantly. The PC\u20102 value changed during the phases in which the change in the PC\u20101 value was unnaturally slow and stopped at CS22 and the first half of CS23. This period, defined as the \u201capproach period\u201d, corresponds to the time before dynamic changes occur as the palatal shelves elevate, the tongue and mandibular tip change their position and shape, and secondary palatal shelves contact and fuse. During the \u201capproach period\u201d, measurements of PC\u20102 changes showed that structures on the mandible (Meckel's cartilage and tongue) and maxilla (palate and nasal cavity) did not change positions, albeit both groups of structures appeared to be compressed anterior\u2013posteriorly. However, during and after shelf elevation, measurements of PC\u20101 changes showed significant changes between maxillary and mandibular structures, particularly positioning of the shelves above the tongue and protrusion of the tongue and mandible. These results suggest an active role for Meckel's cartilage growth in repositioning the tongue to facilitate shelf elevation. The present data representing three distinct phases of secondary palate closure in humans can advance the understanding of morphological growth changes occurring before and after the horizontal positioning of palatal shelves and their fusion to close the secondary palate in humans successfully.<\/jats:p>","DOI":"10.1111\/joa.13745","type":"journal-article","created":{"date-parts":[[2022,8,19]],"date-time":"2022-08-19T09:56:23Z","timestamp":1660902983000},"page":"1287-1302","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Morphometric analysis of secondary palate development in human embryos"],"prefix":"10.1111","volume":"241","author":[{"given":"Aoi","family":"Nohara","sequence":"first","affiliation":[{"name":"Human Health Science Kyoto University Graduate School of Medicine  Kyoto 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