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Rodent NMJs are readily accessible, and research into conditions such as amyotrophic lateral sclerosis (ALS), Charcot\u2013Marie\u2013Tooth disease (CMT), and spinal muscular atrophy (SMA) has relied heavily on experimental work in these small mammals. However, given that nerve length dependency is an important feature of many peripheral neuropathies, these rodent models have clear shortcomings; large animal models might be preferable, but their size presents novel anatomical challenges. Overcoming these constraints to study the NMJ morphology of large mammalian distal limb muscles is of prime importance to increase cross\u2010species translational neuromuscular research potential, particularly in the study of long motor units. In the past, NMJ phenotype analysis of large muscle bodies within the equine distal pelvic limb, such as the <jats:italic>tibialis cranialis<\/jats:italic>, or within muscles of high fibrous content, such as the <jats:italic>soleus<\/jats:italic>, has posed a distinct experimental hurdle. We optimised a technique for NMJ location and dissection from equine pelvic limb muscles. Using a quantification method validated in smaller species, we demonstrate their morphology and show that equine NMJs can be reliably dissected, stained and analysed. We reveal that the NMJs within the equine <jats:italic>soleus<\/jats:italic> have distinctly different morphologies when compared to the <jats:italic>extensor digitorum longus<\/jats:italic> and <jats:italic>tibialis cranialis<\/jats:italic> muscles. Overall, we demonstrate that equine distal pelvic limb muscles can be regionally dissected, with samples whole\u2010mounted and their innervation patterns visualised. These methods will allow the localisation and analysis of neuromuscular junctions within the muscle bodies of large mammals to identify neuroanatomical and neuropathological features.<\/jats:p>","DOI":"10.1111\/joa.13747","type":"journal-article","created":{"date-parts":[[2022,9,10]],"date-time":"2022-09-10T16:03:35Z","timestamp":1662825815000},"page":"1133-1147","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A method to identify, dissect and stain equine neuromuscular junctions for morphological analysis"],"prefix":"10.1111","volume":"241","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2287-1416","authenticated-orcid":false,"given":"Stephen D.","family":"Cahalan","sequence":"first","affiliation":[{"name":"Comparative Neuromuscular Diseases Laboratory, Department of Clinical Science and Services Royal Veterinary College  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin D.","family":"Perkins","sequence":"additional","affiliation":[{"name":"Comparative Neuromuscular Diseases Laboratory, Department of Clinical Science and Services Royal Veterinary College  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9343-0944","authenticated-orcid":false,"given":"Ines","family":"Boehm","sequence":"additional","affiliation":[{"name":"Edinburgh Medical School: Biomedical Sciences University of Edinburgh  Edinburgh UK"},{"name":"Euan MacDonald Centre for Motor Neuron Disease Research University of Edinburgh  Edinburgh UK"},{"name":"Biozentrum University of Basel  Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ross A.","family":"Jones","sequence":"additional","affiliation":[{"name":"Edinburgh Medical School: Biomedical Sciences University of Edinburgh  Edinburgh UK"},{"name":"Euan MacDonald Centre for Motor Neuron Disease Research University of Edinburgh  Edinburgh UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0306-5577","authenticated-orcid":false,"given":"Thomas H.","family":"Gillingwater","sequence":"additional","affiliation":[{"name":"Edinburgh Medical School: Biomedical Sciences University of Edinburgh  Edinburgh UK"},{"name":"Euan MacDonald Centre for Motor Neuron Disease Research University of Edinburgh  Edinburgh UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4344-6438","authenticated-orcid":false,"given":"Richard J.","family":"Piercy","sequence":"additional","affiliation":[{"name":"Comparative Neuromuscular Diseases Laboratory, Department of Clinical Science and Services Royal Veterinary College  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2018.05.008"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","unstructured":"Bianchi L.&Bianchi L.M.(2018)Developmental neurobiology. 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